Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.8.23 extracted from

  • Freeman, S.; Pollack, S.J.; Knowles, J.R.
    Synthesis of the unusual metabolite carboxyphosphoenolpyruvate. Cloning and expression of carboxyphosphonoenolpyruvate mutase (1992), J. Am. Chem. Soc., 114, 377-378.
No PubMed abstract available

Application

Application Comment Organism
agriculture formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production Streptomyces hygroscopicus

Cloned(Commentary)

Cloned (Comment) Organism
cloned and expressd at high levels in Escherichia coli Streptomyces hygroscopicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
carboxyphosphoenolpyruvate
-
Streptomyces hygroscopicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
2 * 32000, homodimer, SDS-PAGE Streptomyces hygroscopicus
32700
-
calculated from amino acid composition Streptomyces hygroscopicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carboxyphosphonoenolpyruvate Streptomyces hygroscopicus
-
(hydroxyphosphinyl)pyruvate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces hygroscopicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxyphosphoenolpyruvate
-
Streptomyces hygroscopicus ?
-
?
carboxyphosphonoenolpyruvate
-
Streptomyces hygroscopicus (hydroxyphosphinyl)pyruvate + CO2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 32000, homodimer, SDS-PAGE Streptomyces hygroscopicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
carboxyphosphoenolpyruvate
-
Streptomyces hygroscopicus