Application | Comment | Organism |
---|---|---|
agriculture | formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production | Streptomyces hygroscopicus |
Cloned (Comment) | Organism |
---|---|
cloned and expressd at high levels in Escherichia coli | Streptomyces hygroscopicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
carboxyphosphoenolpyruvate | - |
Streptomyces hygroscopicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, homodimer, SDS-PAGE | Streptomyces hygroscopicus |
32700 | - |
calculated from amino acid composition | Streptomyces hygroscopicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxyphosphonoenolpyruvate | Streptomyces hygroscopicus | - |
(hydroxyphosphinyl)pyruvate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces hygroscopicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces hygroscopicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxyphosphoenolpyruvate | - |
Streptomyces hygroscopicus | ? | - |
? | |
carboxyphosphonoenolpyruvate | - |
Streptomyces hygroscopicus | (hydroxyphosphinyl)pyruvate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, homodimer, SDS-PAGE | Streptomyces hygroscopicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
carboxyphosphoenolpyruvate | - |
Streptomyces hygroscopicus |