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Literature summary for 2.7.2.2 extracted from

  • Srivenugopal, K.S.; Adiga, P.R.
    Enzymic conversion of agmatine to putrescine in Lathyrus sativus seedlings. Purification and properties of a multifunctional enzyme (putrescine synthase) (1981), J. Biol. Chem., 256, 9532-9541.
    View publication on PubMed

General Stability

General Stability Organism
all the component activities associated with putrescine synthase are stabilized in dilute solutions, 0.05 mg of protein per ml, for about 3-4 h at 37°C by 0.250 mg/ml bovine serum albumin Lathyrus sativus
prolonged dialysis and freeze-thawing, loss of activity Lathyrus sativus
purified enzyme is highly unstable even in presence of glycerol, dithiothreitol and Mg2+ Lathyrus sativus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + NH3 + CO2 Lathyrus sativus multifunctional enzyme, involved in biosynthesis of putrescine ADP + carbamoyl phosphate
-
r

Organism

Organism UniProt Comment Textmining
Lathyrus sativus
-
enzyme has inherent activities of agmatine iminohydrolase, putrescine transcarbamylase, ornithine transcarbamylase and carbamate kinase
-

Purification (Commentary)

Purification (Comment) Organism
enzyme has inherent activities of agmatine iminohydrolase, putrescine transcarbamylase, ornithine transcarbamylase and carbamate kinase Lathyrus sativus

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Lathyrus sativus
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Storage Stability

Storage Stability Organism
4°C, 48 h, complete loss of activity Lathyrus sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + carbamoyl phosphate
-
Lathyrus sativus ATP + NH3 + CO2
-
r
ATP + NH3 + CO2
-
Lathyrus sativus ADP + carbamoyl phosphate
-
r
ATP + NH3 + CO2 multifunctional enzyme, involved in biosynthesis of putrescine Lathyrus sativus ADP + carbamoyl phosphate
-
r

Synonyms

Synonyms Comment Organism
putrescine synthase enzyme has inherent activities of agmatine iminohydrolase, putrescine transcarbamylase, ornithinetranscarbamylase and carbamate kinase Lathyrus sativus