Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Trypanosoma brucei gambiense |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
ADP | at pH 6.8 and 37°C | Trypanosoma brucei gambiense | |
5.54 | - |
sn-glycerol 3-phosphate | at pH 6.8 and 37°C | Trypanosoma brucei gambiense |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60400 | - |
- |
Trypanosoma brucei gambiense |
119000 | - |
gel filtration | Trypanosoma brucei gambiense |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + sn-glycerol 3-phosphate | Trypanosoma brucei gambiense | - |
ATP + glycerol | - |
r | |
ATP + glycerol | Trypanosoma brucei gambiense | - |
ADP + sn-glycerol 3-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei gambiense | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity resin column chromatography and gel filtration | Trypanosoma brucei gambiense |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.4 | - |
lysate, at pH 6.8 and 37°C | Trypanosoma brucei gambiense |
32 | - |
after 76fold purification, at pH 6.8 and 37°C | Trypanosoma brucei gambiense |
Storage Stability | Organism |
---|---|
4°C, in 0.1 M sodium phosphate buffer pH 6.8 containing 10 mM MgSO4, 48 h, more than 50% loss of activity | Trypanosoma brucei gambiense |
4°C, in 0.1 M sodium phosphate buffer pH 6.8 containing 10 mM MgSO4, 5 mM ADP, 10 mM MgSO4 and 0.1% (v/v) glycerol, 4 weeks, no loss of activity | Trypanosoma brucei gambiense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + sn-glycerol 3-phosphate | - |
Trypanosoma brucei gambiense | ATP + glycerol | - |
r | |
ATP + glycerol | - |
Trypanosoma brucei gambiense | ADP + sn-glycerol 3-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 60400, calculated from amino acid sequence | Trypanosoma brucei gambiense |
Synonyms | Comment | Organism |
---|---|---|
ATP:glycerol 3-phosphotransferase | - |
Trypanosoma brucei gambiense |
GLPK | - |
Trypanosoma brucei gambiense |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 70 | - |
Trypanosoma brucei gambiense |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Trypanosoma brucei gambiense |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | the enzyme shows 45-100% over a wide range of pH (5.0-7.0) at 37°C. Activity is almost zero up to pH 4.5, but declines relatively slower in the alkaline side | Trypanosoma brucei gambiense |