Cloned (Comment) | Organism |
---|---|
expression of dak 1 in Escherichia coli | Schizosaccharomyces pombe |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | competitive inhibition | Schizosaccharomyces pombe |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
dihydroxyacetone | pH 7.5, 25°C, recombinant enzyme | Schizosaccharomyces pombe | |
0.63 | - |
ATP | pH 7.5, 25°C, recombinant enzyme | Schizosaccharomyces pombe |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 4 mM, 116% activation | Schizosaccharomyces pombe | |
Co2+ | 4 mM, 20% activation | Schizosaccharomyces pombe | |
Mg2+ | 4 mM, 100% activation | Schizosaccharomyces pombe | |
Mn2+ | 4 mM, 12% activation | Schizosaccharomyces pombe |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
2 * 62000, recombinant enzyme, SDS-PAGE | Schizosaccharomyces pombe |
62245 | - |
2 * 62245, recombinant enzyme, deduced from amino acid sequence | Schizosaccharomyces pombe |
145000 | - |
gel filtration | Schizosaccharomyces pombe |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | O13902 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, butyl-Toyopearl, UNO-Q6, recombinant enzyme | Schizosaccharomyces pombe |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.9 | - |
- |
Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dihydroxyacetone | - |
Schizosaccharomyces pombe | ADP + dihydroxyacetone phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 62000, recombinant enzyme, SDS-PAGE | Schizosaccharomyces pombe |
dimer | 2 * 62245, recombinant enzyme, deduced from amino acid sequence | Schizosaccharomyces pombe |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
ADP | - |
Schizosaccharomyces pombe |