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Literature summary for 2.7.1.29 extracted from

  • Bystrykh, L.V.; de Koning, W.; Harder, W.
    Triokinase from Candida boidinii KD1 (1990), Methods Enzymol., 188, 445-451.
    View publication on PubMed

General Stability

General Stability Organism
glycerol, most effective stabilizer, EDTA and MgCl2 stabilize to a lesser extent, inactivated during ammonium sulfate precipitation [Candida] boidinii

Inhibitors

Inhibitors Comment Organism Structure
Be2+
-
[Candida] boidinii
Ca2+
-
[Candida] boidinii
Co2+
-
[Candida] boidinii
Cu2+
-
[Candida] boidinii
Mn2+
-
[Candida] boidinii
additional information both components of the MgATP complex inhibit the kinase at excess concentration [Candida] boidinii
Zn2+
-
[Candida] boidinii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
ATP pH 8.0, 30-37°C [Candida] boidinii
0.35
-
ATP ATP in form of MgATP2- [Candida] boidinii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity, no other cation can replace Mg2+ [Candida] boidinii
additional information no tightly bound metal ions [Candida] boidinii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
2 * 71000, SDS-PAGE [Candida] boidinii
139000
-
-
[Candida] boidinii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + dihydroxyacetone [Candida] boidinii second step in assimilation of formaldehyde via the xylulose monophosphate, i.e. dihydroxyacetone cycle during growth of yeast on methanol ADP + dihydroxyacetone phosphate
-
?
ATP + dihydroxyacetone [Candida] boidinii KD1 second step in assimilation of formaldehyde via the xylulose monophosphate, i.e. dihydroxyacetone cycle during growth of yeast on methanol ADP + dihydroxyacetone phosphate
-
?

Organism

Organism UniProt Comment Textmining
[Candida] boidinii
-
-
-
[Candida] boidinii KD1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
polyethylene glycol 6000, polyethyleneimine, DEAE-Sepharose, Sepharose CL-6B [Candida] boidinii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.33
-
-
[Candida] boidinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + dihydroxyacetone
-
[Candida] boidinii ADP + dihydroxyacetone phosphate
-
ir
ATP + dihydroxyacetone second step in assimilation of formaldehyde via the xylulose monophosphate, i.e. dihydroxyacetone cycle during growth of yeast on methanol [Candida] boidinii ADP + dihydroxyacetone phosphate
-
?
ATP + dihydroxyacetone
-
[Candida] boidinii KD1 ADP + dihydroxyacetone phosphate
-
ir
ATP + dihydroxyacetone second step in assimilation of formaldehyde via the xylulose monophosphate, i.e. dihydroxyacetone cycle during growth of yeast on methanol [Candida] boidinii KD1 ADP + dihydroxyacetone phosphate
-
?
ATP + DL-glyceraldehyde 25% of the activity with dihydroxyacetone [Candida] boidinii ADP + glyceraldehyde 3-phosphate
-
?
ATP + glycerone
-
[Candida] boidinii ADP + glycerone phosphate
-
?
CTP + dihydroxyacetone 25% of activity with ATP [Candida] boidinii CDP + dihydroxyacetone phosphate
-
?
CTP + dihydroxyacetone 25% of activity with ATP [Candida] boidinii KD1 CDP + dihydroxyacetone phosphate
-
?
GTP + dihydroxyacetone 25% of activity with ATP [Candida] boidinii GDP + dihydroxyacetone phosphate
-
?
GTP + dihydroxyacetone 25% of activity with ATP [Candida] boidinii KD1 GDP + dihydroxyacetone phosphate
-
?
ITP + dihydroxyacetone 25% of activity with ATP [Candida] boidinii IDP + dihydroxyacetone phosphate
-
?
ITP + dihydroxyacetone 25% of activity with ATP [Candida] boidinii KD1 IDP + dihydroxyacetone phosphate
-
?
UTP + dihydroxyacetone 25% of activity with ATP [Candida] boidinii UDP + dihydroxyacetone phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 71000, SDS-PAGE [Candida] boidinii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at [Candida] boidinii
37
-
assay at [Candida] boidinii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.2
-
[Candida] boidinii

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.7 8.7 optimal stability [Candida] boidinii

Cofactor

Cofactor Comment Organism Structure
additional information no tightly bound cofactor [Candida] boidinii