General Stability | Organism |
---|---|
glycerol, most effective stabilizer, EDTA and MgCl2 stabilize to a lesser extent, inactivated during ammonium sulfate precipitation | [Candida] boidinii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Be2+ | - |
[Candida] boidinii | |
Ca2+ | - |
[Candida] boidinii | |
Co2+ | - |
[Candida] boidinii | |
Cu2+ | - |
[Candida] boidinii | |
Mn2+ | - |
[Candida] boidinii | |
additional information | both components of the MgATP complex inhibit the kinase at excess concentration | [Candida] boidinii | |
Zn2+ | - |
[Candida] boidinii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
ATP | pH 8.0, 30-37°C | [Candida] boidinii | |
0.35 | - |
ATP | ATP in form of MgATP2- | [Candida] boidinii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity, no other cation can replace Mg2+ | [Candida] boidinii | |
additional information | no tightly bound metal ions | [Candida] boidinii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
2 * 71000, SDS-PAGE | [Candida] boidinii |
139000 | - |
- |
[Candida] boidinii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dihydroxyacetone | [Candida] boidinii | second step in assimilation of formaldehyde via the xylulose monophosphate, i.e. dihydroxyacetone cycle during growth of yeast on methanol | ADP + dihydroxyacetone phosphate | - |
? | |
ATP + dihydroxyacetone | [Candida] boidinii KD1 | second step in assimilation of formaldehyde via the xylulose monophosphate, i.e. dihydroxyacetone cycle during growth of yeast on methanol | ADP + dihydroxyacetone phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Candida] boidinii | - |
- |
- |
[Candida] boidinii KD1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
polyethylene glycol 6000, polyethyleneimine, DEAE-Sepharose, Sepharose CL-6B | [Candida] boidinii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.33 | - |
- |
[Candida] boidinii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dihydroxyacetone | - |
[Candida] boidinii | ADP + dihydroxyacetone phosphate | - |
ir | |
ATP + dihydroxyacetone | second step in assimilation of formaldehyde via the xylulose monophosphate, i.e. dihydroxyacetone cycle during growth of yeast on methanol | [Candida] boidinii | ADP + dihydroxyacetone phosphate | - |
? | |
ATP + dihydroxyacetone | - |
[Candida] boidinii KD1 | ADP + dihydroxyacetone phosphate | - |
ir | |
ATP + dihydroxyacetone | second step in assimilation of formaldehyde via the xylulose monophosphate, i.e. dihydroxyacetone cycle during growth of yeast on methanol | [Candida] boidinii KD1 | ADP + dihydroxyacetone phosphate | - |
? | |
ATP + DL-glyceraldehyde | 25% of the activity with dihydroxyacetone | [Candida] boidinii | ADP + glyceraldehyde 3-phosphate | - |
? | |
ATP + glycerone | - |
[Candida] boidinii | ADP + glycerone phosphate | - |
? | |
CTP + dihydroxyacetone | 25% of activity with ATP | [Candida] boidinii | CDP + dihydroxyacetone phosphate | - |
? | |
CTP + dihydroxyacetone | 25% of activity with ATP | [Candida] boidinii KD1 | CDP + dihydroxyacetone phosphate | - |
? | |
GTP + dihydroxyacetone | 25% of activity with ATP | [Candida] boidinii | GDP + dihydroxyacetone phosphate | - |
? | |
GTP + dihydroxyacetone | 25% of activity with ATP | [Candida] boidinii KD1 | GDP + dihydroxyacetone phosphate | - |
? | |
ITP + dihydroxyacetone | 25% of activity with ATP | [Candida] boidinii | IDP + dihydroxyacetone phosphate | - |
? | |
ITP + dihydroxyacetone | 25% of activity with ATP | [Candida] boidinii KD1 | IDP + dihydroxyacetone phosphate | - |
? | |
UTP + dihydroxyacetone | 25% of activity with ATP | [Candida] boidinii | UDP + dihydroxyacetone phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 71000, SDS-PAGE | [Candida] boidinii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | [Candida] boidinii |
37 | - |
assay at | [Candida] boidinii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.2 | - |
[Candida] boidinii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.7 | 8.7 | optimal stability | [Candida] boidinii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no tightly bound cofactor | [Candida] boidinii |