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Literature summary for 2.7.1.28 extracted from

  • Frandsen, E.K.; Grunnet, N.
    Kinetic properties of triokinase from rat liver (1971), Eur. J. Biochem., 23, 588-592.
    View publication on PubMed
Search for more literature for 2.7.1.28

Inhibitors

Inhibitors Comment Organism Structure
ADP free ADP, 0.5 mM, 50% inhibition, competitive to MgATP2-, noncompetitive to D-glyceraldehyde Rattus norvegicus
ATP free ATP, 1.5 mM, 50% inhibition, competitive to MgATP2-, noncompetitive to D-glyceraldehyde Rattus norvegicus
MgADP- free, competitive to MgATP2-, noncompetitive to D-glyceraldehyde Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 dihydroxyacetone pH 7.5, 23-24°C, at 2 mM ATPMg2- Rattus norvegicus
0.008
-
 D-glyceraldehyde pH 7.5, 23-24°C, cosubstrate 1.0 mM MgATP2- Rattus norvegicus
0.012
-
 D-glyceraldehyde pH 7.5, 23-24°C, cosubstrate 0.5 mM MgATP2- Rattus norvegicus
0.019
-
 D-glyceraldehyde pH 7.5, 23-24°C, cosubstrate 2.0 mM MgATP2- Rattus norvegicus
0.16
-
 ATP pH 7.5, 23-24°C, 11.2 mM free Mg2+ Rattus norvegicus
0.16
-
 ATP ATP in form of MgATP2- Rattus norvegicus
0.2
-
 ATP pH 7.5, 23-24°C, 1.11 mM free Mg2+ Rattus norvegicus
0.2
-
 ATP ATP in form of MgATP2- Rattus norvegicus
0.24
-
 ATP pH 7.5, 23-24°C, 0.34 mM free Mg2+ Rattus norvegicus
0.24
-
 ATP ATP in form of MgATP2- Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required in equimolar concentration with ATP Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially purified Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glyceraldehyde
-
 Rattus norvegicus ADP + D-glyceraldehyde 3-phosphate
-
 ?
ATP + D-glyceraldehyde
-
 Rattus norvegicus ADP + D-glyceraldehyde-3-phosphate
-
 ?
ATP + dihydroxyacetone 2.5fold higher activity than with D-glyceraldehyde Rattus norvegicus ADP + dihydroxyacetone phosphate
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.38
-
 ATP pH 7.5, 23-24°C Rattus norvegicus
1.1
-
 ADP pH 7.5, 23-24°C Rattus norvegicus