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Literature summary for 2.5.1.72 extracted from

  • Cherrier, M.V.; Chan, A.; Darnault, C.; Reichmann, D.; Amara, P.; Ollagnier de Choudens, S.; Fontecilla-Camps, J.C.
    The crystal structure of Fe4S4 quinolinate synthase unravels an enzymatic dehydration mechanism that uses tyrosine and a hydrolase-type triad (2014), J. Am. Chem. Soc., 136, 5253-5256.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ [4Fe-4S] cluster-containing enzyme Thermotoga maritima
[4Fe-4S] cluster the presence of the [4Fe-4S] cluster generates an internal tunnel and a cavity Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerone phosphate + iminosuccinate Thermotoga maritima
-
pyridine-2,3-dicarboxylate + 2 H2O + phosphate
-
?
glycerone phosphate + iminosuccinate Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
pyridine-2,3-dicarboxylate + 2 H2O + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9X1X7 gene nadA
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 Q9X1X7 gene nadA
-

Reaction

Reaction Comment Organism Reaction ID
glycerone phosphate + iminosuccinate = pyridine-2,3-dicarboxylate + 2 H2O + phosphate the presence of the Fe4S4 cluster generates an internal tunnel and a cavity to bind the substrate and and dehydrate it, which is initiated by the conserved residue Tyr21. Tyr21 is close to a conserved Thr-His-Glu. All of these residues are essential for activity and Tyr21 deprotonation, to form the reactive nucleophilic phenoxide anion, is mediated by the triad. NadA displays a dehydration mechanism significantly different from the one found in archetypical dehydratases such as aconitase, which use a serine residue deprotonated by an oxyanion hole Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerone phosphate + iminosuccinate
-
Thermotoga maritima pyridine-2,3-dicarboxylate + 2 H2O + phosphate
-
?
glycerone phosphate + iminosuccinate
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 pyridine-2,3-dicarboxylate + 2 H2O + phosphate
-
?

Synonyms

Synonyms Comment Organism
Fe4S4 quinolinate synthase
-
Thermotoga maritima
NadA
-
Thermotoga maritima

General Information

General Information Comment Organism
physiological function quinolinate synthase is a Fe4S4 cluster-containing dehydrating enzyme involved in the synthesis of quinolinic acid, the universal precursor of the essential nicotinamide adenine dinucleotide coenzyme Thermotoga maritima