Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | [4Fe-4S] cluster-containing enzyme | Thermotoga maritima | |
[4Fe-4S] cluster | the presence of the [4Fe-4S] cluster generates an internal tunnel and a cavity | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerone phosphate + iminosuccinate | Thermotoga maritima | - |
pyridine-2,3-dicarboxylate + 2 H2O + phosphate | - |
? | |
glycerone phosphate + iminosuccinate | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | - |
pyridine-2,3-dicarboxylate + 2 H2O + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9X1X7 | gene nadA | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | Q9X1X7 | gene nadA | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
glycerone phosphate + iminosuccinate = pyridine-2,3-dicarboxylate + 2 H2O + phosphate | the presence of the Fe4S4 cluster generates an internal tunnel and a cavity to bind the substrate and and dehydrate it, which is initiated by the conserved residue Tyr21. Tyr21 is close to a conserved Thr-His-Glu. All of these residues are essential for activity and Tyr21 deprotonation, to form the reactive nucleophilic phenoxide anion, is mediated by the triad. NadA displays a dehydration mechanism significantly different from the one found in archetypical dehydratases such as aconitase, which use a serine residue deprotonated by an oxyanion hole | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerone phosphate + iminosuccinate | - |
Thermotoga maritima | pyridine-2,3-dicarboxylate + 2 H2O + phosphate | - |
? | |
glycerone phosphate + iminosuccinate | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | pyridine-2,3-dicarboxylate + 2 H2O + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Fe4S4 quinolinate synthase | - |
Thermotoga maritima |
NadA | - |
Thermotoga maritima |
General Information | Comment | Organism |
---|---|---|
physiological function | quinolinate synthase is a Fe4S4 cluster-containing dehydrating enzyme involved in the synthesis of quinolinic acid, the universal precursor of the essential nicotinamide adenine dinucleotide coenzyme | Thermotoga maritima |