Literature summary for 2.4.2.30 extracted from

Ashok, Y.; Miettinen, M.; Oliveira, D.K.H.; Tamirat, M.Z.; Naereoja, K.; Tiwari, A.; Hottiger, M.O.; Johnson, M.S.; Lehtioe, L.; Pulliainen, A.T.
Discovery of compounds inhibiting the ADP-ribosyltransferase activity of pertussis toxin (2020), ACS Infect. Dis., 6, 588-602 .

Search for more literature for 2.4.2.30

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Bordetella pertussis

Protein Variants

Protein Variants	Comment	Organism
Q127D/E129D	the mutant shows drastically diminished activity compared to the wild type enzyme	Bordetella pertussis

Inhibitors

Inhibitors	Comment	Organism	Structure
(1E,4E)-1,5-di(pyridin-3-yl)penta-1,4-dien-3-one	i.e. NSC149286	Bordetella pertussis
4,5,7,9-tetrahydro-2H-purine-2,8(3H)-dithione	i.e. NSC29193	Bordetella pertussis
4-nitro-7-[(1-oxo-1lambda(5)-pyridin-2-yl)sulfanyl]-2,1,3-benzoxadiazole	i.e. NSC228155	Bordetella pertussis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
NAD+ + Galphai	Bordetella pertussis	-	nicotinamide + (ADP-D-ribosyl)-Galphai + H+	-	?
NAD+ + Galphai	Bordetella pertussis Tohama I	-	nicotinamide + (ADP-D-ribosyl)-Galphai + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Bordetella pertussis	P04977	-	-
Bordetella pertussis Tohama I	P04977	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography and Superdex 75 gel filtration	Bordetella pertussis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NAD+ + Galphai	-	Bordetella pertussis	nicotinamide + (ADP-D-ribosyl)-Galphai + H+	-	?
NAD+ + Galphai	-	Bordetella pertussis Tohama I	nicotinamide + (ADP-D-ribosyl)-Galphai + H+	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 23400, recombinant C-terminally truncated enzyme, SDS-PAGE	Bordetella pertussis
monomer	1 * 30000, native enzyme, SDS-PAGE	Bordetella pertussis

Synonyms

Synonyms	Comment	Organism
ADP-ribosyltransferase	-	Bordetella pertussis
ART	-	Bordetella pertussis
pertussis toxin	-	Bordetella pertussis
PtxS1	-	Bordetella pertussis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
54.7	-	melting temperature	Bordetella pertussis

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.003	-	at pH 7.5 and 25°C	Bordetella pertussis	4-nitro-7-[(1-oxo-1lambda(5)-pyridin-2-yl)sulfanyl]-2,1,3-benzoxadiazole
0.0068	-	at pH 7.5 and 25°C	Bordetella pertussis	4,5,7,9-tetrahydro-2H-purine-2,8(3H)-dithione
0.02	-	at pH 7.5 and 25°C	Bordetella pertussis	(1E,4E)-1,5-di(pyridin-3-yl)penta-1,4-dien-3-one

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)