Activating Compound | Comment | Organism | Structure |
---|---|---|---|
protein 14-3-3 | dependent on | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
expression of ExoS in HeLa cells leads to a loss of phosphorylation of ERM proteins, overview, expression of wild-type and mutant ExoS in enzyme-deficient strain PA103, co-expression with 14-3-3 | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a truncated ExoS mutant DELTA51-72 | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas aeruginosa | ExoS is a virulence factor of the pathogen | ? | - |
? | |
NAD+ + (ADP-D-ribosyl)n-acceptor | Pseudomonas aeruginosa | - |
nicotinamide + (ADP-D-ribosyl)n+1-acceptor | - |
? | |
NAD+ + moesin | Pseudomonas aeruginosa | ADP-ribosylated-moesin is a poor target for phosphorylation by protein kinase C and Rho kinase, which shows that ADP-ribosylation directly inhibits ERM phosphorylation | nicotinamide + (ADP-D-ribosyl)-moesin | - |
? | |
NAD+ + Ras GTPase | Pseudomonas aeruginosa | - |
nicotinamide + (ADP-D-ribosyl)-Ras GTPase | - |
? | |
NAD+ + vimentin | Pseudomonas aeruginosa | - |
nicotinamide + (ADP-D-ribosyl)-vimentin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | ExoS is a virulence factor of the pathogen | Pseudomonas aeruginosa | ? | - |
? | |
additional information | ExoS is a bifunctional toxin with an N-terminal Rho GTPase activating protein, GAP, domain, and a C-terminal ADP-ribosyltransferase domain which transfers ADP-ribose from NAD onto substrates such as the Ras GTPases and vimentin | Pseudomonas aeruginosa | ? | - |
? | |
NAD+ + (ADP-D-ribosyl)n-acceptor | - |
Pseudomonas aeruginosa | nicotinamide + (ADP-D-ribosyl)n+1-acceptor | - |
? | |
NAD+ + moesin | ADP-ribosylated-moesin is a poor target for phosphorylation by protein kinase C and Rho kinase, which shows that ADP-ribosylation directly inhibits ERM phosphorylation | Pseudomonas aeruginosa | nicotinamide + (ADP-D-ribosyl)-moesin | - |
? | |
NAD+ + moesin | modification of Arg553, Arg560 and Arg563, activity with wild-type and mutant moesins, overview | Pseudomonas aeruginosa | nicotinamide + (ADP-D-ribosyl)-moesin | - |
? | |
NAD+ + Ras GTPase | - |
Pseudomonas aeruginosa | nicotinamide + (ADP-D-ribosyl)-Ras GTPase | - |
? | |
NAD+ + vimentin | - |
Pseudomonas aeruginosa | nicotinamide + (ADP-D-ribosyl)-vimentin | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the N-terminus with amino acids 96219 encodes a Rho GTPase activating protein domain, the C-terminus with amino acids 234453 encodes a 14-3-3-dependent ADP-ribosyltransferase domain | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
ADP-ribosyltransferase | - |
Pseudomonas aeruginosa |
ExoS | - |
Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Pseudomonas aeruginosa |