Cloned (Comment) | Organism |
---|---|
catalytic domain DELTAN22 and DELTAN222 mutants, expression in Escherichia coli | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
DELTAN222 | the carboxy-terminal 222 amino acids, catalytic domain | Pseudomonas aeruginosa |
DELTAN222/E381A | possesses 0.02% of the ADP-ribosyltransferase activity of DELTAN222. KM-value for (ADP-D-ribosyl)n-soybean-trypsin-inhibitor is 8.7fold higher than the KM-value for DELTAN222 | Pseudomonas aeruginosa |
DELTAN222/E381D | possesses 0.6% of the ADP-ribosyltransferase activity of DELTAN222. KM-value for (ADP-D-ribosyl)n-soybean-trypsin-inhibitor is 7.9fold higher than the KM-value for DELTAN222 | Pseudomonas aeruginosa |
DELTAN222/E381S | possesses 0.01% of the ADP-ribosyltransferase activity of DELTAN222. KM-value for (ADP-D-ribosyl)n-soybean-trypsin-inhibitor is 4.7fold higher than the KM-value for DELTAN222 | Pseudomonas aeruginosa |
DELTAN222/E387A | possesses 31% of the ADP-ribosyltransferase activity of DELTAN222 | Pseudomonas aeruginosa |
DELTAN222/E399A | possesses 28% of the ADP-ribosyltransferase activity of DELTAN222 | Pseudomonas aeruginosa |
DELTAN222/E414A | possesses 20% of the ADP-ribosyltransferase activity of DELTAN222 | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Pseudomonas aeruginosa | |
0.049 | - |
(ADP-D-ribosyl)n-soybean-trypsin-inhibitor | DELTAN222 | Pseudomonas aeruginosa | |
0.23 | - |
(ADP-D-ribosyl)n-soybean-trypsin-inhibitor | DELTAN222/E381S | Pseudomonas aeruginosa | |
0.387 | - |
(ADP-D-ribosyl)n-soybean-trypsin-inhibitor | DELTAN222/E381D | Pseudomonas aeruginosa | |
0.429 | - |
(ADP-D-ribosyl)n-soybean-trypsin-inhibitor | DELTAN222/E381A | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant catalytic domain DELTAN22 and DELTAN222 mutants, expression in Escherichia coli | Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
-20°C, 40% glycerol, stable for several months | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (ADP-D-ribosyl)n-soybean-trypsin-inhibitor | - |
Pseudomonas aeruginosa | nicotinamide + (ADP-D-ribosyl)n+1-soybean-trypsin-inhibitor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
exoenzyme S | - |
Pseudomonas aeruginosa |