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Literature summary for 2.4.1.B84 extracted from

  • Gurung, R.B.; Kim, E.H.; Oh, T.J.; Sohng, J.K.
    Enzymatic synthesis of apigenin glucosides by glucosyltransferase (YjiC) from Bacillus licheniformis DSM 13 (2013), Mol. Cells, 36, 355-361 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.B84

Cloned(Commentary)

Cloned (Comment) Organism
gene yjiC, cloned from genomic DNA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis Q65JC2
-
-
Bacillus licheniformis DSM 13 Q65JC2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 UDP-D-glucose + apigenin
-
 Bacillus licheniformis 2 UDP + apigenin 4',7-O-diglucoside
-
 ?
2 UDP-D-glucose + apigenin
-
 Bacillus licheniformis DSM 13 2 UDP + apigenin 4',7-O-diglucoside
-
 ?
additional information enzyme catalyzes glycosylation at the phenolic groups of C-4' and C-7 positions ensuing apigenin 4'-O-glucoside, apigenin 7-O-glucoside and apigenin 4',7-diglucoside as the products leaving the C-5 position unglucosylated Bacillus licheniformis ?
-
-
additional information enzyme catalyzes glycosylation at the phenolic groups of C-4' and C-7 positions ensuing apigenin 4'-O-glucoside, apigenin 7-O-glucoside and apigenin 4',7-diglucoside as the products leaving the C-5 position unglucosylated Bacillus licheniformis DSM 13 ?
-
-
UDP-D-glucose + apigenin
-
 Bacillus licheniformis UDP + apigenin 4'-O-glucoside
-
 ?
UDP-D-glucose + apigenin
-
 Bacillus licheniformis DSM 13 UDP + apigenin 4'-O-glucoside
-
 ?

Synonyms

Synonyms Comment Organism
YjiC
-
 Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.8 formation of apigenin 4',7-di-O-glucoside is preferred Bacillus licheniformis
8.8
-
 formation of apigenin 4'-O-glucoside is preferred Bacillus licheniformis