Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | at higher concentrations, 30 mM: 50% inhibition | Sus scrofa | |
additional information | not inhibited by UTP | Sus scrofa | |
UDP | competitive | Sus scrofa | |
UMP | uncompetitive | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km: 0.83 mg/ml: deglycosylated Cowper's gland mucin, 1.12 mg/ml: deglycosylated human trachea mucin, 0.94 mg/ml: deglycosylated swine trachea mucin, values for several tryptic peptides derived from them | Sus scrofa | |
0.0063 | - |
UDP-GalNAc | - |
Sus scrofa | |
0.0141 | - |
deglycosylated swine trachea mucin | - |
Sus scrofa | |
0.0175 | - |
deglycosylated human trachea mucin | - |
Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | Sus scrofa | 16020 | - |
microsome | - |
Sus scrofa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | requirement | Sus scrofa | |
Mn2+ | maximal activity at 5-7.5 mM, higher concentrations are inhibitory | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
gel filtration | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-galactosamine + polypeptide | Sus scrofa | primary step in the production of mucin-type O-linked oligosaccharide groups in glycoproteins | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography on deglycosylated Cowper's gland mucin bound to Sepharose, 12500fold | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tracheal epithelium | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.12 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-80°C, washed mucosal membranes, no loss of activity after 1 year | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | not: UDP-glucuronate, UDP-xylose, GDP-mannose, GDP-fucose, intact mucins, desialylated mucins, glycolipid acceptors, including globoside and ceramide hexosides, no transfer of GalNAc to terminal sugar residues in oligosaccharide chains | Sus scrofa | ? | - |
? | |
additional information | not: UDP-GlcNAc | Sus scrofa | ? | - |
? | |
additional information | not: UDP-Gal | Sus scrofa | ? | - |
? | |
UDP-N-acetyl-alpha-D-galactosamine + deglycosylated human trachea mucin | - |
Sus scrofa | ? | - |
? | |
UDP-N-acetyl-alpha-D-galactosamine + deglycosylated swine trachea mucin | - |
Sus scrofa | ? | - |
? | |
UDP-N-acetyl-D-galactosamine + apomucin | deglycosylated Cowper's gland, swine and human trachea mucins | Sus scrofa | UDP + N-acetyl-D-galactosaminyl-apomucin | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | only UDP-GalNAc serves as sugar donor | Sus scrofa | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | transfer of GalNAc to a serine or threonine residue on the acceptor protein | Sus scrofa | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | acceptors: deglycosylated Cowper's gland, swine and human trachea mucins, tryptic peptides prepared from them, best substrates are fragments of incompletely deglycosylated mucin polypeptide chains | Sus scrofa | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | primary step in the production of mucin-type O-linked oligosaccharide groups in glycoproteins | Sus scrofa | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 70000, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | 7.8 | broad pH-optimum between pH 6.7-7.8 with maximum at pH 7.2 | Sus scrofa |
7.2 | - |
broad pH-optimum between pH 6.7-7.8 with maximum at pH 7.2 | Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
UDP | - |
Sus scrofa | |
0.041 | - |
UMP | - |
Sus scrofa |