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Literature summary for 2.4.1.4 extracted from
- Thermostable amylosucrase from Calidithermus timidus DSM 17022 insight into its characteristics and tetrameric conformation (2019), J. Agric. Food Chem., 67, 9868-9876 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21(DE3) | Calidithermus timidus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65220 | - |
calculated from sequence | Calidithermus timidus |
| 264950 | - |
gel filtration | Calidithermus timidus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Calidithermus timidus | WP_018466847 | i.e. Calidithermus timidus | - |
| Calidithermus timidus DSM 17022 | WP_018466847 | i.e. Calidithermus timidus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable in solution for over 1 month | Calidithermus timidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose | alpha-1,4-glucan is the predominant product at pH 6.0-8.0 and 30-60°C | Calidithermus timidus | D-fructose + alpha-1,4-glucan | - |
? |  |
| sucrose | alpha-1,4-glucan is the predominant product at pH 6.0-8.0 and 30-60°C | Calidithermus timidus DSM 17022 | D-fructose + alpha-1,4-glucan | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 66000, SDS-PAGE | Calidithermus timidus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ASASE | - |
Calidithermus timidus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
polymerization activity | Calidithermus timidus |
| 55 | - |
total enzyme activity and hydrolysis activity | Calidithermus timidus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 65 | over 60% relative activity between 30°C and 65°C | Calidithermus timidus |
| 40 | 60 | more than 80% relative activity between 40°C and 60°C | Calidithermus timidus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life: 2773 min | Calidithermus timidus |
| 65 | - |
half-life: 1429 min | Calidithermus timidus |
| 70 | - |
half-life: 1.09 h | Calidithermus timidus |
| 74.47 | - |
melting temperature | Calidithermus timidus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
total enzyme activity, polymerization activity and hydrolysis activity | Calidithermus timidus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.5 | pH 5.0: about 50% of maximal activity, pH 7.5: about 60% of maximal activity | Calidithermus timidus |
| 6 | 7.5 | pH 6.0: about 55% of maximal activity, pH 7.5: about 45% of maximal activity, polymerization activity | Calidithermus timidus |
| 6 | 7.5 | pH 6.0: about 60% of maximal activity, pH 7.5: about 50% of maximal activity, total activity | Calidithermus timidus |
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Release 2023.1 (January 2023)
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