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Literature summary for 2.4.1.4 extracted from
- Identification of an alpha-(1,4)-glucan-synthesizing amylosucrase from Cellulomonas carboniz T26 (2017), J. Agric. Food Chem., 65, 2110-2119 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed in Escherchia coli | Cellulomonas carbonis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 71934 | - |
calculated from sequence | Cellulomonas carbonis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellulomonas carbonis | A0A0A0BUC7 | - |
- |
| Cellulomonas carbonis T26 | A0A0A0BUC7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel affinity chromatography | Cellulomonas carbonis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1100 | - |
pH 5.0, 40°C, sucrose hydrolysis | Cellulomonas carbonis |
| 4200 | - |
pH 7.0, 40°C, transglucosylation reaction | Cellulomonas carbonis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose | transglucosylation activity is significantly higher than the hydrolytic activity. The main product generated from sucrose is structurally determined to be alpha-(1,4)-glucan. A small amount of glucose is produced by hydrolysis, and sucrose isomers including turanose and trehalulose are generated as minor products. The ratio of hydrolytic, polymerization, and isomerization reactions is calculated to be 5.8:84.0:10.2. The enzyme favors production of long-chain insoluble alpha-glucan at lower temperature | Cellulomonas carbonis | alpha-glucan + turanose + trehalulose | - |
? |  |
| sucrose | transglucosylation activity is significantly higher than the hydrolytic activity. The main product generated from sucrose is structurally determined to be alpha-(1,4)-glucan. A small amount of glucose is produced by hydrolysis, and sucrose isomers including turanose and trehalulose are generated as minor products. The ratio of hydrolytic, polymerization, and isomerization reactions is calculated to be 5.8:84.0:10.2. The enzyme favors production of long-chain insoluble alpha-glucan at lower temperature | Cellulomonas carbonis T26 | alpha-glucan + turanose + trehalulose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 70000, SDS-PAGE | Cellulomonas carbonis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
transglucosylation activity | Cellulomonas carbonis |
| 45 | - |
hydrolytic activity | Cellulomonas carbonis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
retains more than 90% of its initial activity after incubation for 58 h | Cellulomonas carbonis |
| 45 | - |
loses more than 50% of its initial activity after incubation for 1 h | Cellulomonas carbonis |
| 50 | - |
loses more than 50% of its initial activity after incubation for 5 min | Cellulomonas carbonis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
transglucosylation activity | Cellulomonas carbonis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Cellulomonas carbonis | calculated from sequence | - |
5.39 |
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Release 2023.1 (January 2023)
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