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Literature summary for 2.4.1.344 extracted from

  • Zhao, C.; Wu, Y.; Yu, H.; Shah, I.; Li, Y.; Zeng, J.; Liu, B.; Mills, D.; Chen, X.
    One-pot multienzyme (OPME) synthesis of human blood group H antigens and a human milk oligosaccharide (HMOS) with highly active Thermosynechococcus elongatus alpha1-2-fucosyltransferase (2016), Chem. Commun. (Camb.), 52, 3899-3902 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.344

Application

Application Comment Organism
synthesis enzyme is applied in an efficient one-pot multienzyme fucosylation system for high-yield synthesis of human blood group H antigens containing beta1-3-linked galactosides and an important human milk oligosaccharide lacto-N-fucopentaose I in preparative and gram scales Thermosynechococcus vestitus

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermosynechococcus vestitus

Inhibitors

Inhibitors Comment Organism Structure
DTT 10 mM, slight inhibition Thermosynechococcus vestitus
EDTA 10 mM, slight inhibition Thermosynechococcus vestitus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.56
-
 GDP-beta-L-fucose donor hydrolysis reaction, pH not specified in the publication, temperature not specified in the publication Thermosynechococcus vestitus
0.73
-
 GDP-beta-L-fucose fucosyl transferase reaction, pH not specified in the publication, temperature not specified in the publication Thermosynechococcus vestitus
0.79
-
 beta-D-Gal-(1->3)-beta-D-GlcNAc-2-anthranilic acid aglycone pH not specified in the publication, temperature not specified in the publication Thermosynechococcus vestitus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 5-20 mM, about 2fold increase in activity Thermosynechococcus vestitus
Mn2+ 5-20 mM, about 2fold increase in activity Thermosynechococcus vestitus
additional information no divalnet cation required Thermosynechococcus vestitus

Organism

Organism UniProt Comment Textmining
Thermosynechococcus vestitus Q8DK72
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-L-fucose + beta-D-Gal-(1->3)-beta-D-GlcNAc-2-anthranilic acid aglycone
-
 Thermosynechococcus vestitus GDP + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-beta-D-GlcNAc-2-anthranilic acid aglycone
-
 ?
additional information enzyme works well with type I (Galbeta1-3GlcNAcbetaOR) and its derivative (Galbeta1-3GlcNAcalphaOR), Type III (Galbeta1-3GalNAcalphaOR), and type IV (Galbeta1-3GalNAcbetaOR) acceptors for adding a fucose alpha1-2-linked to the terminal galactose residue. Type II (Galbeta1-4GlcNAcbetaOR) and type V (Galbeta1-4GlcbetaOR) glycans are not good acceptors Thermosynechococcus vestitus ?
-
-

Synonyms

Synonyms Comment Organism
tll0994
-
 Thermosynechococcus vestitus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 45
-
 Thermosynechococcus vestitus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 GDP-beta-L-fucose donor hydrolysis reaction, pH not specified in the publication, temperature not specified in the publication Thermosynechococcus vestitus
0.333
-
 beta-D-Gal-(1->3)-beta-D-GlcNAc-2-anthranilic acid aglycone pH not specified in the publication, temperature not specified in the publication Thermosynechococcus vestitus
0.483
-
 GDP-beta-L-fucose fucosyl transferase reaction, pH not specified in the publication, temperature not specified in the publication Thermosynechococcus vestitus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 fucosyl donor hydrolysis activity is minimum in the pH range 5.0-8.0. An optimal pH at 9.5 is observed for the GDP-fucose hydrolysis activity Thermosynechococcus vestitus
4.5 6 broad Thermosynechococcus vestitus

pH Range

pH Minimum pH Maximum Comment Organism
6.5
-
 about 85% of maximum activity Thermosynechococcus vestitus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.009
-
 GDP-beta-L-fucose donor hydrolysis reaction, pH not specified in the publication, temperature not specified in the publication Thermosynechococcus vestitus
0.422
-
 beta-D-Gal-(1->3)-beta-D-GlcNAc-2-anthranilic acid aglycone pH not specified in the publication, temperature not specified in the publication Thermosynechococcus vestitus
0.602
-
 GDP-beta-L-fucose fucosyl transferase reaction, pH not specified in the publication, temperature not specified in the publication Thermosynechococcus vestitus