Cloned (Comment) | Organism |
---|---|
KanF cannot be functionally expressed in Escherichia coli | Streptomyces kanamyceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
2-deoxystreptamine | using UDP-D-glucose as donor, in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C | Streptomyces kanamyceticus | |
0.32 | - |
2-deoxystreptamine | using UDP-N-acetyl-alpha-D-glucosamine as donor, in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C | Streptomyces kanamyceticus | |
0.44 | - |
UDP-D-glucose | in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl22, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C | Streptomyces kanamyceticus | |
2.54 | - |
UDP-N-acetyl-alpha-D-glucosamine | in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C | Streptomyces kanamyceticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces kanamyceticus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-glucose + 2-deoxystreptamine | best substrate | Streptomyces kanamyceticus | UDP + 2'-deamino-2'-hydroxyparomamine | - |
? | |
UDP-N-acetyl-alpha-D-glucosamine + 2-deoxystreptamine | reaction of 2.4.1.283, one fifth of the catalytic efficiency with UDP-D-glucose | Streptomyces kanamyceticus | UDP + 2'-N-acetylparomamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
kanF | - |
Streptomyces kanamyceticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
UDP-D-glucose | in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C | Streptomyces kanamyceticus | |
0.33 | - |
UDP-N-acetyl-alpha-D-glucosamine | in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C | Streptomyces kanamyceticus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
UDP-N-acetyl-alpha-D-glucosamine | in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C | Streptomyces kanamyceticus | |
0.66 | - |
UDP-D-glucose | in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C | Streptomyces kanamyceticus |