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Literature summary for 2.4.1.266 extracted from

  • Alarico, S.; Nunes-Costa, D.; Silva, A.; Costa, M.; Macedo-Ribeiro, S.; Empadinhas, N.
    A genuine mycobacterial thermophile Mycobacterium hassiacum growth, survival and GpgS stability at near-pasteurization temperatures (2020), Microbiology, 166, 474-483 .
    View publication on PubMed
Search for more literature for 2.4.1.266

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycolicibacterium hassiacum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ GpgS is dependent on divalent cations in the decreasing order of efficiency: Mg2+ (20 mM) > Mn2+ (10 mM) Mycolicibacterium hassiacum

Organism

Organism UniProt Comment Textmining
Mycolicibacterium hassiacum K5B7Z4
-
-
Mycolicibacterium hassiacum DSM 44199 K5B7Z4
-
-

Storage Stability

Storage Stability Organism
0°C, one month, no loss of activity Mycolicibacterium hassiacum
23°C, one week, no loss of activity Mycolicibacterium hassiacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-glucose + 3-phospho-D-glycerate
-
 Mycolicibacterium hassiacum ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
ADP-glucose + 3-phospho-D-glycerate
-
 Mycolicibacterium hassiacum DSM 44199 ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
GDP-glucose + 3-phospho-D-glycerate
-
 Mycolicibacterium hassiacum GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
GDP-glucose + 3-phospho-D-glycerate
-
 Mycolicibacterium hassiacum DSM 44199 GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
additional information cosubstrate efficiency in decreasing order: UDP-glucose,ADP-glucose and GDP-glucose. No cosubstrate: GDP-mannose Mycolicibacterium hassiacum ?
-
-
additional information cosubstrate efficiency in decreasing order: UDP-glucose,ADP-glucose and GDP-glucose. No cosubstrate: GDP-mannose Mycolicibacterium hassiacum DSM 44199 ?
-
-
UDP-glucose + 3-phospho-D-glycerate
-
 Mycolicibacterium hassiacum UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
UDP-glucose + 3-phospho-D-glycerate
-
 Mycolicibacterium hassiacum DSM 44199 UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?

Subunits

Subunits Comment Organism
? x * 33600, calculated from sequence Mycolicibacterium hassiacum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 half-life 7.8 days Mycolicibacterium hassiacum
50
-
 half-life 6.4 days Mycolicibacterium hassiacum
60
-
 5 min, loss of activity Mycolicibacterium hassiacum
63.5
-
 melting temperature, pH 7.5, in presence of substrates Mycolicibacterium hassiacum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9
-
 Mycolicibacterium hassiacum