Cloned (Comment) | Organism |
---|---|
Homo sapiens hyaluronan synthase 1 is cloned into the plasmid pFLAG-CMV2, for in vitro translation the pF3A WG(BYDV) Flexi vector is used | Homo sapiens |
Streptococcus equisimilis hyaluronan synthase is cloned into the plasmid pKK223-3 for expression in Escherichia coli SURE cells | Streptococcus dysgalactiae subsp. equisimilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan] | Streptococcus dysgalactiae subsp. equisimilis | - |
UDP + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan] | - |
? | |
UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan] | Homo sapiens | - |
UDP + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan] | - |
? | |
UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan] | Streptococcus dysgalactiae subsp. equisimilis | - |
UDP + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan] | - |
? | |
UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan] | Homo sapiens | - |
UDP + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Streptococcus dysgalactiae subsp. equisimilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on a Ni2+-nitrilotriacetic acid resin | Streptococcus dysgalactiae subsp. equisimilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan] | - |
Streptococcus dysgalactiae subsp. equisimilis | UDP + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan] | - |
? | |
UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan] | - |
Homo sapiens | UDP + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan] | - |
? | |
UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan] | - |
Streptococcus dysgalactiae subsp. equisimilis | UDP + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan] | - |
? | |
UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan] | - |
Homo sapiens | UDP + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HAS | - |
Streptococcus dysgalactiae subsp. equisimilis |
HAS | - |
Homo sapiens |
HAS1 | - |
Homo sapiens |
HsHAS1 | - |
Homo sapiens |
hyaluronan synthase | - |
Streptococcus dysgalactiae subsp. equisimilis |
hyaluronan synthase | - |
Homo sapiens |
seHAS | - |
Streptococcus dysgalactiae subsp. equisimilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
HAS capture assay, overnight at room temperature | Streptococcus dysgalactiae subsp. equisimilis |
22 | - |
HAS capture assay, overnight at room temperature | Homo sapiens |
30 | - |
standard assay for HAS activity | Streptococcus dysgalactiae subsp. equisimilis |
37 | - |
standard assay for HAS activity | Homo sapiens |
60 | - |
HAS capture assay, 3 h | Streptococcus dysgalactiae subsp. equisimilis |
60 | - |
HAS capture assay, 3 h | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
HAS capture assay | Streptococcus dysgalactiae subsp. equisimilis |
5 | - |
HAS capture assay | Homo sapiens |