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Literature summary for 2.4.1.19 extracted from

  • Chen, S.; Li, Z.; Gu, Z.; Hong, Y.; Cheng, L.; Holler, T.P.; Li, C.
    Leu600 mutations decrease product inhibition of the beta-cyclodextrin glycosyltransferase from Bacillus circulans STB01 (2018), Int. J. Biol. Macromol., 115, 1194-1201 .
    View publication on PubMed
Search for more literature for 2.4.1.19

Protein Variants

Protein Variants Comment Organism
L600E the mutant shows wild type cyclization activities. The mutation decreases the product inhibition exhibited by beta-cyclodextrin as compared to the wild type enzyme Niallia circulans
L600I the mutant shows decreased cyclization activities compared to the wild type enzyme. The mutation decreases the product inhibition exhibited by beta-cyclodextrin as compared to the wild type enzyme Niallia circulans
L600R the mutant shows increased cyclization activities compared to the wild type enzyme. The mutation decreases the product inhibition exhibited by beta-cyclodextrin as compared to the wild type enzyme Niallia circulans
L600Y the mutant shows wild type cyclization activities. The mutation decreases the product inhibition exhibited by beta-cyclodextrin as compared to the wild type enzyme Niallia circulans

Inhibitors

Inhibitors Comment Organism Structure
beta-cyclodextrin product inhibition, mixed-type inhibition Niallia circulans

Organism

Organism UniProt Comment Textmining
Niallia circulans A0A097CPM3
-
-
Niallia circulans STB01 A0A097CPM3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltodextrin + glycosyl acceptor 38% conversion Niallia circulans beta-cyclodextrin + gamma-cyclodextrin + alpha-cyclodextrin
-
 ?
maltodextrin + glycosyl acceptor 38% conversion Niallia circulans STB01 beta-cyclodextrin + gamma-cyclodextrin + alpha-cyclodextrin
-
 ?

Synonyms

Synonyms Comment Organism
beta-cyclodextrin glycosyltransferase
-
 Niallia circulans
CGTase
-
 Niallia circulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
280
-
 maltodextrin mutant enzyme L600Y, at pH 6.5 and 50°C Niallia circulans
282
-
 maltodextrin wild type enzyme, at pH 6.5 and 50°C Niallia circulans
285
-
 maltodextrin mutant enzyme L600I, at pH 6.5 and 50°C Niallia circulans
299
-
 maltodextrin mutant enzyme L600E, at pH 6.5 and 50°C Niallia circulans
358
-
 maltodextrin mutant enzyme L600R, at pH 6.5 and 50°C Niallia circulans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.05
-
 beta-cyclodextrin wild type enzyme, at pH 6.5 and 50°C Niallia circulans
0.06
-
 beta-cyclodextrin mutant enzyme L600Y, at pH 6.5 and 50°C Niallia circulans
0.19
-
 beta-cyclodextrin mutant enzyme L600E, at pH 6.5 and 50°C Niallia circulans
0.28
-
 beta-cyclodextrin mutant enzyme L600I, at pH 6.5 and 50°C Niallia circulans
0.5
-
 beta-cyclodextrin mutant enzyme L600R, at pH 6.5 and 50°C Niallia circulans