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Literature summary for 2.4.1.19 extracted from
- Domain shuffling of cyclodextrin glucanotransferases for tailored product specificity and thermal stability (2019), FEBS Open Bio, 9, 384-395 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Geobacillus stearothermophilus |
| expressed in Escherichia coli BL21(DE3) cells | Bacillus sp. G-825-6 |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 10 mM used in assay conditions | Geobacillus stearothermophilus |  |
| Mg2+ | 10 mM used in assay conditions | Bacillus sp. G-825-6 | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. G-825-6 | - |
- |
- |
| Geobacillus stearothermophilus | - |
- |
- |
| Geobacillus stearothermophilus ET1/NO2 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| soluble starch + glycosyl acceptor | - |
Bacillus sp. G-825-6 | gamma-cyclodextrin | - |
? | |
| soluble starch + glycosyl acceptor | - |
Geobacillus stearothermophilus | alpha-cyclodextrin + beta-cyclodextrin | - |
? | |
| soluble starch + glycosyl acceptor | - |
Geobacillus stearothermophilus ET1/NO2 | alpha-cyclodextrin + beta-cyclodextrin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CGTase | - |
Geobacillus stearothermophilus |
| CGTase | - |
Bacillus sp. G-825-6 |
| cyclodextrin glucanotransferase | - |
Geobacillus stearothermophilus |
| cyclodextrin glucanotransferase | - |
Bacillus sp. G-825-6 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | most active | Bacillus sp. G-825-6 |
| 70 | 80 | most active | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 71 | - |
melting temperature | Bacillus sp. G-825-6 |
| 88 | - |
melting temperature | Geobacillus stearothermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | most active | Geobacillus stearothermophilus |
| 8 | 10 | most active | Bacillus sp. G-825-6 |
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Release 2023.1 (January 2023)
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