Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Paenibacillus macerans |
Protein Variants | Comment | Organism |
---|---|---|
A156V | the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme | Paenibacillus macerans |
A156V/A166Y | the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme | Paenibacillus macerans |
A156V/L174P | the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme | Paenibacillus macerans |
A156V/L174P/A166Y | the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme | Paenibacillus macerans |
A166Y | the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme | Paenibacillus macerans |
A166Y/L174P | the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme | Paenibacillus macerans |
L174P | the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme | Paenibacillus macerans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus macerans | P04830 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Paenibacillus macerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
genistein + maltodextrin | less than 20% conversion ratio | Paenibacillus macerans | alpha-cyclodextrins | - |
? | |
sophoricoside + maltodextrin | more than 40% conversion ratio | Paenibacillus macerans | Glc-sophoricoside + Glc2-sophoricoside + Glc3-sophoricoside + Glc4-sophoricoside + Glc5-sophoricoside + Glc6-sophoricoside | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 75000, SDS-PAGE | Paenibacillus macerans |
Synonyms | Comment | Organism |
---|---|---|
CGTase | - |
Paenibacillus macerans |
cyclodextrin glucosyltransferase | - |
Paenibacillus macerans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
sophoricoside | wild type enzyme, at pH 6.0 and 50°C | Paenibacillus macerans | |
0.063 | - |
sophoricoside | mutant enzyme A156V/L174P/A166Y, at pH 6.0 and 50°C | Paenibacillus macerans | |
0.079 | - |
maltodextrin | wild type enzyme, at pH 6.0 and 50°C | Paenibacillus macerans | |
0.079 | - |
maltodextrin | mutant enzyme A156V/L174P/A166Y, at pH 6.0 and 50°C | Paenibacillus macerans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Paenibacillus macerans |