Cloned (Comment) | Organism |
---|---|
expression of cyclodextrin glycosyltransferase gene fused with thioredoxin, hexa-histidine and S-protein at the N-terminus and a proline-rich peptide at the C-terminus, in Escherichia coli | Paenibacillus sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of cyclodextrin glycosyltransferase gene fused with thioredoxin, hexa-histidine and S-protein at the N-terminus and a proline-rich peptide at the C-terminus, in Escherichia coli. The maximum specific activity for enzyme is 2.7fold higher than that of the non-fusion form, leading to a specific activity of 2268 units/mg protein at a 61% yield. The fusion enzyme is superior than its wild-type counterpart in terms of stability against high temperature and organic solvents. The fusion enzyme catalyzes the synthesis of cyclodextrins in 20% v/v dimethylformamide with a higher product yield of cyclodextrins CD7 and CD8 compared to that of the wild-type enzyme in the same buffer-solvent system | Paenibacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. | - |
- |
- |
Paenibacillus sp. A11 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant fusion protein | Paenibacillus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2268 | - |
glycosyltransferase gene fused with thioredoxin, hexa-histidine and S-protein at the N-terminus and a proline-rich peptide at the C-terminus, pH 6.0, 40°C | Paenibacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
soluble potoato starch | - |
Paenibacillus sp. | cyclodextrin | - |
? | |
soluble potoato starch | - |
Paenibacillus sp. A11 | cyclodextrin | - |
? |