General Stability | Organism |
---|---|
loss of activity on Q-Sepharose during ion exchange chromatography | Neurospora crassa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Neurospora crassa | |
phosphate | slight inhibition at 100 mM | Neurospora crassa | |
UDP | inhibits both autoglucosylation and glucosylation of exogenous acceptor | Neurospora crassa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | can not substitute Mn2+ | Neurospora crassa | |
Mn2+ | dependent on, also required for transglucosylation | Neurospora crassa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
SDS-PAGE | Neurospora crassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + glycogenin | Escherichia coli | enzyme forms the protein part of proteoglycogen | UDP + glucosylated glycogenin | - |
? | |
UDP-glucose + glycogenin | Neurospora crassa | enzyme forms the protein part of proteoglycogen | UDP + glucosylated glycogenin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Neurospora crassa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | autoglycosylation | Escherichia coli |
glycoprotein | autoglycosylation | Neurospora crassa |
Purification (Comment) | Organism |
---|---|
separation from proteoglycogen | Neurospora crassa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin | independent active sites for glucosylation of exogenous and self-acceptors | Neurospora crassa | |
UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin | highly conserved protein | Escherichia coli | |
UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin | highly conserved protein | Neurospora crassa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Neurospora crassa | - |
Storage Stability | Organism |
---|---|
-20°C, amylolyzed M-glycogenin preparation, at least 1 month without appreciable loss of activity | Neurospora crassa |
4°C, amylolyzed M-glycogenin preparation, at least 1 week without appreciable loss of activity | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + glycogenin | autoglycosylation reaction | Escherichia coli | UDP + glucosylated glycogenin | forms glucosyl-alpha1,4-glucosyl linkage | ? | |
UDP-glucose + glycogenin | autoglycosylation reaction | Neurospora crassa | UDP + glucosylated glycogenin | forms glucosyl-alpha1,4-glucosyl linkage | ? | |
UDP-glucose + glycogenin | enzyme forms the protein part of proteoglycogen | Escherichia coli | UDP + glucosylated glycogenin | - |
? | |
UDP-glucose + glycogenin | enzyme forms the protein part of proteoglycogen | Neurospora crassa | UDP + glucosylated glycogenin | - |
? | |
UDP-glucose + glycogenin | enzyme forms the protein part of proteoglycogen | Escherichia coli | UDP + glucosylated glycogenin | forms glucosyl-alpha1,4-glucosyl linkage | ? | |
UDP-glucose + glycogenin | enzyme forms the protein part of proteoglycogen | Neurospora crassa | UDP + glucosylated glycogenin | forms glucosyl-alpha1,4-glucosyl linkage | ? | |
UDP-glucose + glycogenin | UDP-glucose can not be replaced by ADP- or GDP-glucose | Neurospora crassa | UDP + glucosylated glycogenin | forms glucosyl-alpha1,4-glucosyl linkage | ? | |
UDP-glucose + n-dodecyl-beta-D-maltoside | transglucosylation reaction | Escherichia coli | UDP + n-dodecyl-beta-D-maltotriose | - |
? | |
UDP-glucose + n-dodecyl-beta-D-maltoside | transglucosylation reaction | Neurospora crassa | UDP + n-dodecyl-beta-D-maltotriose | - |
? | |
UDP-glucose + n-dodecyl-beta-D-maltoside | simultaneously and independently of the autoglycosylation reaction | Escherichia coli | UDP + n-dodecyl-beta-D-maltotriose | - |
? | |
UDP-glucose + n-dodecyl-beta-D-maltoside | simultaneously and independently of the autoglycosylation reaction | Neurospora crassa | UDP + n-dodecyl-beta-D-maltotriose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE | Neurospora crassa |
More | enzyme forms the protein part of proteoglycogen | Escherichia coli |
More | enzyme forms the protein part of proteoglycogen | Neurospora crassa |
Synonyms | Comment | Organism |
---|---|---|
M-glycogenin | - |
Escherichia coli |
M-glycogenin | - |
Neurospora crassa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Neurospora crassa |