KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
UDP-glucose | 4-hydroxymandelonitrile or 3,4-dihydroxymandelonitrile | Triglochin maritima |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Triglochin maritima | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
High salt concentrations | 160% activity observed at 0.6 M concentration of NaCl, KCl or ammonium sulfate with 4-hydroxymandelonitrile, and 20% activity observed at 0.1-0.2 M concentration with 3,4-dihydroxymandelonitrile | Triglochin maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
1 * 47000, SDS-PAGE | Triglochin maritima |
50000 | - |
gel filtration | Triglochin maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 4-hydroxymandelonitrile | Triglochin maritima | last step in biosynthesis of cyanogenic glucosides | UDP + taxiphyllin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triglochin maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
method that includes chromatography on Sephadex PD10 and DEAE-Sephadex | Triglochin maritima |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Triglochin maritima | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.31 | - |
3,4-dihydroxymandelonitrile | Triglochin maritima |
0.94 | - |
4-hydroxymandelonitrile | Triglochin maritima |
Storage Stability | Organism |
---|---|
-20°C, 10% glycerol, crude extract stable for several weeks | Triglochin maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 3,4-dihydroxymandelonitrile | - |
Triglochin maritima | UDP + 3,4-dihydroxymandelonitrile glucoside | - |
? | |
UDP-glucose + 4-hydroxymandelonitrile | - |
Triglochin maritima | UDP + taxiphyllin | - |
? | |
UDP-glucose + 4-hydroxymandelonitrile | last step in biosynthesis of cyanogenic glucosides | Triglochin maritima | UDP + taxiphyllin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47000, SDS-PAGE | Triglochin maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Triglochin maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | - |
Triglochin maritima |
8 | - |
assay at | Triglochin maritima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | about 50% of activity maximum at pH 6 and pH 9.5 | Triglochin maritima |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
unstable below, crude enzyme extract | Triglochin maritima |