Cloned (Comment) | Organism |
---|---|
genetic interaction between dGlcAT-P and dC1GalT1, recombinant expression of dGlcAT-I, dGlcAT-P-II, and dGlcAT-S-I in Spodoptera frugiperda Sf21 insect cells | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
additional information | creation of dGlcAT-P null mutants, mutant larvae show lower expression of glucuronylated T antigen on the muscles and at NMJs. Mislocalization of neuromuscular junction (NMJ) boutons and a partial loss of the basement membrane components collagen IV (Col IV) and nidogen (Ndg) at the muscle 6/7 boundary are observed. Those two phenotypes are correlated and identical to previously described phenotypes in dC1GalT1 mutant larvae. In addition, dGlcAT-P null mutants exhibit fewer NMJ branches on muscles 6/7. Ultrastructural analysis reveals that basement membranes that lacks Col IV and Ndg are significantly deformed. The loss of dGlcAT-P expression causes ultrastructural defects in NMJ boutons | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
basement membrane | a specialized extracellular matrix that underlies the epithelium and surrounds other tissues, including muscles, fat, and nerve fibers | Drosophila melanogaster | 5604 | - |
additional information | immunohistochemic analysis | Drosophila melanogaster | - |
- |
neuromuscular junction | NMJ | Drosophila melanogaster | 31594 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucuronate + [protein]-3-O-(beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl)-L-serine | Drosophila melanogaster | - |
UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | M9NE76 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Drosophila melanogaster | - |
hemocyte | - |
Drosophila melanogaster | - |
larva | - |
Drosophila melanogaster | - |
additional information | immunohistochemic analysis | Drosophila melanogaster | - |
muscle fibre | - |
Drosophila melanogaster | - |
S2 cell | - |
Drosophila melanogaster | - |
wing disc | larval | Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | analysis of glycan structure by mass spectrometry. Activity analysis of enzyme splicing variants, overview | Drosophila melanogaster | ? | - |
- |
|
UDP-alpha-D-glucuronate + [protein]-3-O-(beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl)-L-serine | - |
Drosophila melanogaster | UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase | UniProt | Drosophila melanogaster |
GlcAT-P | - |
Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Drosophila melanogaster |
General Information | Comment | Organism |
---|---|---|
malfunction | dGlcAT-P null mutants larvae show lower expression of glucuronylated T antigen on the muscles and at neuromuscular junctions (NMJs). Mislocalization of NMJ boutons and a partial loss of the basement membrane components collagen IV (Col IV) and nidogen (Ndg) at the muscle 6/7 boundary are observed. The phenotypes correlate to previously described phenotypes in dC1GalT1 mutant larvae. dGlcAT-P null mutants exhibit fewer NMJ branches on muscles 6/7 compared to wild-type. Basement membranes that lack Col IV and Ndg are significantly deformed. The loss of dGlcAT-P expression causes ultrastructural defects in NMJ boutons. Genetic interaction between dGlcAT-P and dC1GalT1 is determined. Phenotypes of dGlcAT-P mutants, detailed overview | Drosophila melanogaster |
metabolism | genetic interaction between dGlcAT-P and dC1GalT1 (EC 2.4.1.122), and glucuronylated T antigen, rather than unmodified T antigen, contributes to precise localization of NMJ boutons and normal formation of basement membranes on muscles 6/7. Glucuronylated core 1 glycans synthesized by dGlcAT-P are key modulators of neuromuscular junction (NMJ) bouton localization, basement membrane formation, and NMJ arborization on larval muscles. In Drosophila, three major mucin-type O-glycan structures, i.e. Tn antigen (GalNAcalpha1-Ser/Thr), unmodified T antigen (core 1 or Galbeta1-3GalNAcalpha1-Ser/Thr), and glucuronylated T antigen (glucuronylated core 1 or GlcAbeta1-3Galbeta1-3GalNAcalpha1-Ser/Thr), have been reported. And three Drosophila beta1,3-glucuronyltransferases (dGlcATs), dGlcAT-I (DmGlcAT-I), dGlcAT-S (DmGlcAT-BSI), and dGlcAT-P (DmGlcAT-BSII), have been reported in Drosophila. Both dGlcAT-P and dGlcAT-S can transfer GlcA to T antigen in vitro, whereas only dGlcAT-P modifies T antigen in S2 cells | Drosophila melanogaster |
physiological function | the major components of basement membrane are proteins modified by glycans. In Drosophila, glucuronylation of T antigen is predominantly carried out by Drosophila beta1,3-glucuronyltransferase-P (dGlcAT-P). T antigen formation occurs mainly due to the activity of Drosophila C1GalT1 orthologue | Drosophila melanogaster |