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Literature summary for 2.3.3.17 extracted from

  • Textor, S.; Bartram, S.; Kroymann, J.; Falk, K.L.; Hick, A.; Pickett, J.A.; Gershenzon, J.
    Biosynthesis of methionine-derived glucosinolates in Arabidopsis thaliana: recombinant expression and characterization of methylthioalkylmalate synthase, the condensing enzyme of the chain-elongation cycle (2004), Planta, 218, 1026-1035.
    View publication on PubMed
Search for more literature for 2.3.3.17

Activating Compound

Activating Compound Comment Organism Structure
ADP the addition of ADP increases the rate of reaction over 10fold Arabidopsis thaliana
ATP the addition of 20 mM ATP increases the rate of reaction over 10fold Arabidopsis thaliana
dithiothreitol required for activity Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition at 0.2 mM Arabidopsis thaliana
iodoacetamide effective inhibitor, complete inhibition at 1 mM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.245
-
 acetyl-CoA at pH 8.5 and 32°C Arabidopsis thaliana
0.64
-
 5-methylthio-2-oxopentanoate at pH 8.5 and 32°C Arabidopsis thaliana
3
-
 4-methylthio-2-oxobutanoate at pH 8.5 and 32°C Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates nearly equivalent to Mn2+ Arabidopsis thaliana
Fe2+ activates nearly equivalent to Mn2+ Arabidopsis thaliana
Mn2+ optimal activity at 4 mM Arabidopsis thaliana
additional information Cu2+ and Zn2+ do not support catalysis at all Arabidopsis thaliana
Ni2+ activates nearly equivalent to Mn2+ Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 1 * 50000, SDS-PAGE Arabidopsis thaliana
51200
-
-
 Arabidopsis thaliana
52000
-
 gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an omega-(methylthio)-2-oxoalkanoate + acetyl-CoA + H2O Arabidopsis thaliana
-
 a 2-[omega-(methylthio)alkyl]malate + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FG67 ecotype Columbia
-

Purification (Commentary)

Purification (Comment) Organism
phenyl Sepharose column chromatography and Superdex 200 gel filtration Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoheptanoate + acetyl-CoA + H2O 84% activity compared to 5-methylthio-2-oxopentanoate Arabidopsis thaliana 2-pentylmalate + CoA
-
 ?
2-oxohexanoate + acetyl-CoA + H2O 17% activity compared to 5-methylthio-2-oxopentanoate Arabidopsis thaliana 2-butylmalate + CoA
-
 ?
4-methylthio-2-oxobutanoate + acetyl-CoA + H2O 86% activity compared to 5-methylthio-2-oxopentanoate Arabidopsis thaliana 2-(2'-methylthioethyl)malate + CoA
-
 ?
5-methylthio-2-oxopentanoate + acetyl-CoA + H2O 100% activity Arabidopsis thaliana 2-(3'-methylthiopropyl)malate + CoA
-
 ?
an omega-(methylthio)-2-oxoalkanoate + acetyl-CoA + H2O
-
 Arabidopsis thaliana a 2-[omega-(methylthio)alkyl]malate + CoA
-
 ?
additional information no activity with 6-methylthio-2-oxohexanoate, 2-oxopentanoate, 2-oxooctanoate, glyoxylate, pyruvate, 2-oxoisovalerate, 2-phenylpyruvate, oxaloacetate, 2-oxoglutarate, and 2-oxoadipate Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Arabidopsis thaliana
monomer 1 * 51200, calculated from amino acid sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
MAM1 isoform Arabidopsis thaliana
MAMS
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
-
 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
7.4 9.7 50% activity at pH 7.4 and 9.7 Arabidopsis thaliana