Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C41(DE3) cells | Thermoplasma acidophilum |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M CHES pH 9.5, 12% (w/v) PEG 4000, 1 mM oxaloacetate | Thermoplasma acidophilum |
Protein Variants | Comment | Organism |
---|---|---|
H222Q | the mutant shows less than 0.05% of wild type activity | Thermoplasma acidophilum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ACMX | competitive inhibitor versus acetyl-CoA | Thermoplasma acidophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
acetyl-CoA | pH and temperature not specified in the publication | Thermoplasma acidophilum | |
0.0052 | - |
oxaloacetate | pH and temperature not specified in the publication | Thermoplasma acidophilum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | Thermoplasma acidophilum | - |
citrate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma acidophilum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-loaded iminodiacetic acid Sepharose column chromatography and Sephadex 200 gel filtration | Thermoplasma acidophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | - |
Thermoplasma acidophilum | citrate + CoA | - |
? |