Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermoplasma acidophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
free enzyme forms various binary and ternary complexes with substrates | Thermoplasma acidophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
crystalline | - |
Thermoplasma acidophilum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from E. coli | Thermoplasma acidophilum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + H2O + oxaloacetate = citrate + CoA | active site | Sus scrofa | |
acetyl-CoA + H2O + oxaloacetate = citrate + CoA | active site | Thermoplasma acidophilum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | condensation step nearly irreversible, reversibility is increased at 70°C | Thermoplasma acidophilum | citrate + CoA | - |
r | |
acetyl-CoA + oxaloacetate + H2O | condensation step is reversible | Sus scrofa | citrate + CoA | - |
r |
Synonyms | Comment | Organism |
---|---|---|
CS | - |
Sus scrofa |
CS | - |
Thermoplasma acidophilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Sus scrofa |
70 | - |
- |
Thermoplasma acidophilum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kcat is stable over pH-range 6.0-8.0 | Thermoplasma acidophilum | |
additional information | - |
additional information | kinetics | Sus scrofa | |
additional information | - |
additional information | kinetics | Thermoplasma acidophilum | |
additional information | - |
additional information | kcat with citrate and CoA | Sus scrofa | |
additional information | - |
additional information | kcat with citrate and CoA | Thermoplasma acidophilum | |
9.17 | - |
acetyl-CoA | recombinant wild-type, pH 8.0 | Thermoplasma acidophilum | |
9.17 | - |
citrate | recombinant enzyme, pH 8.0 | Thermoplasma acidophilum | |
117 | - |
citrate | - |
Sus scrofa | |
167 | - |
acetyl-CoA | pH 7.5 | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 8.9 | Thermoplasma acidophilum |