Cloned (Comment) | Organism |
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expression in Escherichia coli as either His-tagged enzyme with three different signal peptides and in fusions with three different signal peptides and in fusion with thioredoxin, glutathione S-transferase, and (pre-)maltose-binding protein. In all cases, the expressed protein is either undetectable or insoluble. Expression in Pichia pastoris of the enzyme fused to the alpha-factor leader results in low levels of activity. Extracellular expression of the enzyme in the insect cell/baculovirus system is sucessfull. Enzyme N-terminally fused to a combined secretion signal/His-tag peptide is correctly processed by the host signal peptidase and the His-tag can subsequently be removed with dipeptidyl peptidase I | Carica papaya |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carica papaya | O81226 | - |
- |