General Stability | Organism |
---|---|
EDTA, 1-2 mM, stabilizes | Cavia porcellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | 1 mol per mol enzyme, 85% inactivation, not reversed by glutathione | Cavia porcellus | |
iodoacetamide | 0.1 mM, pH 6.8, in the presence of Ca2+, complete inhibition, irreversible, incorporation of 1 mol carbamidomethyl/mol enzyme, substrate protects | Cavia porcellus | |
N-ethylmaleimide | 3.6 mol per mol enzyme, 91% inhibition in the presence of Ca2+, not inhibited in the absence of Ca2+, substrate protects | Cavia porcellus | |
p-chloromercuribenzoate | reversible by glutathione | Cavia porcellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
66 | - |
carbobenzoxy-L-glutaminylglycine | - |
Cavia porcellus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Cavia porcellus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | requirement | Cavia porcellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76900 | - |
sedimentation and diffusion | Cavia porcellus |
86000 | 94000 | sedimentation equilibrium | Cavia porcellus |
90000 | - |
sedimentation equilibrium, meniscus depletion method, iodoacetamide-incorporation studies | Cavia porcellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
Hartley strain | - |
Cavia porcellus Hartley | - |
Hartley strain | - |
Purification (Comment) | Organism |
---|---|
- |
Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Cavia porcellus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.5 | - |
- |
Cavia porcellus |
Storage Stability | Organism |
---|---|
4°C, 5 mM Tris-HCl/2 mM EDTA buffer, pH 7.5, up to 3 months | Cavia porcellus |