Cloned (Comment) | Organism |
---|---|
recombinant protein fused to glutathione-S-transferase is expressed in | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
caffeoyl-CoA | pH 6.6, 30°C, cosubstrate: shikimate | Nicotiana tabacum | |
0.15 | - |
p-Coumaroyl-CoA | pH 6.6, 30°C, cosubstrate: quinate | Nicotiana tabacum | |
0.35 | - |
feruloyl-CoA | pH 6.6, 30°C, cosubstrate: shikimate | Nicotiana tabacum | |
0.6 | - |
p-Coumaroyl-CoA | pH 6.6, 30°C, cosubstrate: shikimate | Nicotiana tabacum | |
0.73 | - |
caffeoyl-CoA | pH 6.6, 30°C, cosubstrate: quinate | Nicotiana tabacum | |
0.75 | - |
shikimate | pH 6.6, 30°C, cosubstrate: p-coumaroyl-CoA | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Nicotiana tabacum | the enzyme controls shikimate and quinate ester intermediates in phenylpropanoid metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | Q8GSM7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein fused to glutathione-S-transferase | Nicotiana tabacum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stem | - |
Nicotiana tabacum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
decrease in absorbance at DELTA346 nM | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
caffeoyl-CoA + quinate | - |
Nicotiana tabacum | CoA + 5-O-caffeoylquinate | - |
? | |
caffeoyl-CoA + shikimate | caffeoyl-CoA is the most efficient acyl group donor with shikimate as substrate | Nicotiana tabacum | CoA + 5-O-caffeoylshikimate | - |
? | |
feruloyl-CoA + shikimate | feruloyl-CoA shows 10fold lower activity than caffeoyl-CoA with shikimate as substrate | Nicotiana tabacum | CoA + 5-O-feruloylshikimate | - |
? | |
additional information | the enzyme controls shikimate and quinate ester intermediates in phenylpropanoid metabolism | Nicotiana tabacum | ? | - |
? | |
additional information | no activity is detected with quinate and feruloyl-CoA or sinapoyl-CoA | Nicotiana tabacum | ? | - |
? | |
p-coumaroyl-CoA + quinate | 4-coumaroyl-CoA is the most efficient acyl group donor with quinate as substrate | Nicotiana tabacum | CoA + 5-O-(p-coumaroyl)quinate | - |
? | |
p-coumaroyl-CoA + shikimate | - |
Nicotiana tabacum | CoA + 5-O-(p-coumaroyl)shikimate | - |
? |