Application | Comment | Organism |
---|---|---|
synthesis | development of a methodology that utilizes aminoglycoside acetyltransferase and unnatural acyl-CoA analogues for the chemoenzymatic generation of regioselectively N-acylatedaminoglycoside analogues. Aminoglycosides are broad-spectrum antibiotics commonly used for the treatment of serious bacterial infections | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expression of C- or N-terminally His-tagged enzyme from vectors pET22b, and pET28a or Int-pET19b-pps, respectively | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00057 | - |
sisomicin | pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.00096 | - |
amikacin | pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.0015 | - |
sisomicin | pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.002 | - |
kanamycin A | pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET28a | Escherichia coli | |
0.0021 | - |
amikacin | pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.0027 | - |
tobramycin | pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.0029 | - |
gentamicin | pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.0031 | - |
neomycin B | pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.0033 | - |
kanamycin A | pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps | Escherichia coli | |
0.0046 | - |
gentamicin | pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.0048 | - |
kanamycin A | pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET28a | Escherichia coli | |
0.0054 | - |
tobramycin | pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.0055 | - |
kanamycin A | pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.0091 | - |
kanamycin A | pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps | Escherichia coli | |
0.0099 | - |
kanamycin A | pH 6.6, with acetyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps | Escherichia coli | |
0.0111 | - |
kanamycin A | pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.0135 | - |
kanamycin A | pH 6.6, with n-propionyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps | Escherichia coli | |
0.0148 | - |
neomycin B | pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.0189 | - |
gentamicin | pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.0716 | - |
amikacin | pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-bromo-thiophene-2-carbonyl-S-CoA + amikacin | - |
Escherichia coli | ? | - |
? | |
4-bromo-thiophene-2-carbonyl-S-CoA + gentamicin | - |
Escherichia coli | ? | - |
? | |
4-bromo-thiophene-2-carbonyl-S-CoA + neomycin B | - |
Escherichia coli | ? | - |
? | |
4-bromo-thiophene-2-carbonyl-S-CoA + paromomycin | - |
Escherichia coli | ? | - |
? | |
4-bromo-thiophene-2-carbonyl-S-CoA + tobramycin | - |
Escherichia coli | ? | - |
? | |
5-bromo-thiophene-2-carbonyl-S-CoA + kanamycin A | - |
Escherichia coli | ? | - |
? | |
5-bromo-thiophene-2-carbonyl-S-CoA + neomycin B | - |
Escherichia coli | ? | - |
? | |
6-fluoropicolinyl-CoA + neomycin B | - |
Escherichia coli | ? | - |
? | |
acetyl-CoA + amikacin | - |
Escherichia coli | CoA + N3'-acetyl-amikacin | - |
? | |
acetyl-CoA + gentamicin | - |
Escherichia coli | CoA + N3'-acetyl-gentamicin | - |
? | |
acetyl-CoA + kanamycin A | - |
Escherichia coli | CoA + N3'-acetylkanamycin A | - |
? | |
acetyl-CoA + neomycin B | - |
Escherichia coli | CoA + N3-acetyl-neomycin B | - |
? | |
acetyl-CoA + paromomycin | - |
Escherichia coli | CoA + N3'-acetyl-paromomycin | - |
? | |
acetyl-CoA + sisomicin | - |
Escherichia coli | CoA + N3'-acetyl-sisomicin | - |
? | |
acetyl-CoA + tobramycin | - |
Escherichia coli | CoA + N3'-acetyl-tobramycin | - |
? | |
butyryl-CoA + kanamycin A | - |
Escherichia coli | CoA + N3'-butyryl-kanamycin A | - |
? | |
glutaryl-CoA + kanamycin A | - |
Escherichia coli | ? | - |
? | |
glycyl-CoA + amikacin | - |
Escherichia coli | CoA + N3'-glycyl-amikacin | - |
? | |
glycyl-CoA + gentamicin | - |
Escherichia coli | CoA + N3'-glycyl-gentamicin | - |
? | |
glycyl-CoA + kanamycin A | - |
Escherichia coli | CoA + N3'-glycyl-kanamycin A | - |
? | |
glycyl-CoA + neomycin B | - |
Escherichia coli | CoA + N3-glycylneomycin B | - |
? | |
glycyl-CoA + sisomicin | - |
Escherichia coli | CoA + N3'-glycyl-sisomicin | - |
? | |
malonyl-CoA + amikacin | - |
Escherichia coli | CoA + N3'-malonyl-amikacin | - |
? | |
malonyl-CoA + gentamicin | - |
Escherichia coli | CoA + N3'-malonyl-gentamicin | - |
? | |
malonyl-CoA + neomycin B | - |
Escherichia coli | CoA + N3-malonylneomycin B | - |
? | |
methylmalonyl-CoA + neomycin B | - |
Escherichia coli | CoA + N3-methylmalonylneomycin B | - |
? | |
additional information | no activity with benzoyl-CoA, acetoacetyl-CoA, crotonyl-CoA, isovaleryl-CoA, palmitoyl-CoA, and DL-3-hydroxy-butyryl-CoA by AAC(6')-APH(2'') | Escherichia coli | ? | - |
? | |
N-propionyl-CoA + amikacin | - |
Escherichia coli | CoA + N3'-propionyl-amikacin | - |
? | |
N-propionyl-CoA + gentamicin | - |
Escherichia coli | CoA + N3'-propionyl-gentamicin | - |
? | |
N-propionyl-CoA + kanamycin A | - |
Escherichia coli | CoA + N3'-propionyl-kanamycin A | - |
? | |
N-propionyl-CoA + neomycin B | - |
Escherichia coli | CoA + N3-propionylneomycin B | - |
? | |
N-propionyl-CoA + paromomycin | - |
Escherichia coli | CoA + N3'-propionyl-paromomycin | - |
? | |
N-propionyl-CoA + sisomicin | - |
Escherichia coli | CoA + N3'-propionyl-sisomicin | - |
? | |
N-propionyl-CoA + tobramycin | - |
Escherichia coli | CoA + N3'-propionyl-tobramycin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AAC | - |
Escherichia coli |
AAC(3)-IV | - |
Escherichia coli |
AAC(6')-APH(2'') | - |
Escherichia coli |
aminoglycoside acetyltransferase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
kanamycin A | pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET28a | Escherichia coli | |
0.017 | - |
kanamycin A | pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET28a | Escherichia coli | |
0.041 | - |
gentamicin | pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.044 | - |
kanamycin A | pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps | Escherichia coli | |
0.076 | - |
neomycin B | pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.089 | - |
tobramycin | pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.121 | - |
sisomicin | pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.123 | - |
gentamicin | pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.124 | - |
amikacin | pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.126 | - |
amikacin | pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.134 | - |
kanamycin A | pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.144 | - |
kanamycin A | pH 6.6, with n-propionyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps | Escherichia coli | |
0.146 | - |
amikacin | pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.163 | - |
kanamycin A | pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps | Escherichia coli | |
0.173 | - |
sisomicin | pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.179 | - |
tobramycin | pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.182 | - |
gentamicin | pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.188 | - |
neomycin B | pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.3 | - |
kanamycin A | pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b | Escherichia coli | |
0.559 | - |
kanamycin A | pH 6.6, with acetyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
assay at | Escherichia coli |