Literature summary for 2.3.1.47 extracted from

Webster, S.P.; Alexeev, D.; Campopiano, D.J.; Watt, R.M.; Alexeeva, M.; Sawyer, L.; Baxter, R.L.
Mechanism of 8-amino-7-oxononanoate synthase: spectroscopic, kinetic, and crystallographic studies (2000), Biochemistry, 39, 516-528.

Search for more literature for 2.3.1.47

Cloned(Commentary)

Cloned (Comment)	Organism
bioF gene overexpression in Escherichia coli	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
Mg2+ added for structure analysis of the holoenzyme	Escherichia coli
pH 8.5-8.9, ammonium sulfate precipitation, apoenzyme, X-ray diffraction structure analysis with and without bound pyridoxyl-5'-phosphate, model, active site residues	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	concentration above 10 mM of Ca2+, Mg2+, and Mn2+ cause slow precipitation of the enzyme	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.025	-	pimeloyl-CoA	-	Escherichia coli
0.5	-	L-alanine	-	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
41740	-	electrospray mass spectrometry, holoenzyme	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6-carboxyhexanoyl-CoA + L-alanine	Escherichia coli	i.e. pimeloyl-CoA	8-amino-7-oxononanoate + CoA + CO2	i.e. 7-oxo-8-amino-pelargonic acid	ir
6-carboxyhexanoyl-CoA + L-alanine	Escherichia coli	reaction in biotin biosynthesis	8-amino-7-oxononanoate + CoA + CO2	i.e. 7-oxo-8-amino-pelargonic acid	ir

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant from E. coli	Escherichia coli

Reaction

Reaction	Comment	Organism	Reaction ID
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]	reaction mechanism	Escherichia coli	a
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]	binding study of L-alanine and D-alanine to the enzyme	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6-carboxyhexanoyl-CoA + L-alanine	i.e. pimeloyl-CoA	Escherichia coli	8-amino-7-oxononanoate + CoA + CO2	i.e. 7-oxo-8-amino-pelargonic acid	ir
6-carboxyhexanoyl-CoA + L-alanine	reaction in biotin biosynthesis	Escherichia coli	8-amino-7-oxononanoate + CoA + CO2	i.e. 7-oxo-8-amino-pelargonic acid	ir

Synonyms

Synonyms	Comment	Organism
AONS	-	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.001	-	pimeloyl-CoA	-	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	pyridoxal-5'-phosphate binding site	Escherichia coli

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Release 2023.1 (January 2023)