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Literature summary for 2.3.1.47 extracted from

  • Webster, S.P.; Campopiano, D.J.; Alexeev, D.; Alexeeva, M.; Watt, R.M.; Sawyer, L.; Baxter, R.L.
    Characterization of 8-amino-7-oxononanoate synthase: a bacterial PLP-dependent, acyl CoA condensing enzyme (1998), Biochem. Soc. Trans., 26, S268.
    View publication on PubMed
Search for more literature for 2.3.1.47

Cloned(Commentary)

Cloned (Comment) Organism
bioF gene, expression in strain HMS174 (DE3) Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
pH 8.5 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 pimeloyl-CoA
-
 Escherichia coli
0.9
-
 L-alanine
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-carboxyhexanoyl-CoA + L-alanine Escherichia coli i.e. pimeloyl-CoA 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
6-carboxyhexanoyl-CoA + L-alanine Lysinibacillus sphaericus i.e. pimeloyl-CoA 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
6-carboxyhexanoyl-CoA + L-alanine Escherichia coli reaction in biotin biosynthesis 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
6-carboxyhexanoyl-CoA + L-alanine Lysinibacillus sphaericus reaction in biotin biosynthesis 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 gene bioF
-
Lysinibacillus sphaericus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from overexpressing E. coli strain HMS174 (DE3) Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] reaction mechanism Escherichia coli
a
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] reaction mechanism Lysinibacillus sphaericus
a
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] reaction mechanism, formation of the pyridoxal-phosphate-stabilized carbanion by abstraction of the C2-H proton of the alanine-pyridoxal-phosphate aldimine, followed by acylation and decarboxylation Lysinibacillus sphaericus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-carboxyhexanoyl-CoA + L-alanine i.e. pimeloyl-CoA Escherichia coli 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
6-carboxyhexanoyl-CoA + L-alanine i.e. pimeloyl-CoA Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
6-carboxyhexanoyl-CoA + L-alanine reaction in biotin biosynthesis Escherichia coli 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
6-carboxyhexanoyl-CoA + L-alanine reaction in biotin biosynthesis Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?

Subunits

Subunits Comment Organism
More homodimer with each monomer consisting of 3 domains Escherichia coli

Synonyms

Synonyms Comment Organism
AONS
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000833
-
 pimeloyl-CoA
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Escherichia coli
pyridoxal 5'-phosphate
-
 Lysinibacillus sphaericus