Application | Comment | Organism |
---|---|---|
agriculture | possible target for design of inhibitors as herbicides and antibacterial agents | Lysinibacillus sphaericus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-amino-3-hydroxy-2-methylnonadioic acid | competitive, transition state analogue | Lysinibacillus sphaericus | |
4-carboxybutyl(1-amino-1-carboxyethyl)phosphonate | competitive, transition state analogue | Lysinibacillus sphaericus | |
8-amino-7-oxo-8-phosphonononanoic acid | strong inhibition | Lysinibacillus sphaericus | |
D-alanine | competitive; Schiff base formation between D- or L-alanine and pyridoxal-5'-phophate in the active site | Lysinibacillus sphaericus | |
additional information | inhibition mechanism; no inhibition by 8-amino-7-oxo-8-phosphonononanoic acid | Lysinibacillus sphaericus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-carboxyhexanoyl-CoA + L-alanine | Lysinibacillus sphaericus | i.e. pimeloyl-CoA | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ir | |
6-carboxyhexanoyl-CoA + L-alanine | Lysinibacillus sphaericus | second step in biotin biosynthesis | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ir | |
6-carboxyhexanoyl-CoA + L-alanine | Lysinibacillus sphaericus | reaction in biotin biosynthesis | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from E. coli overproducing strain | Lysinibacillus sphaericus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] | binding study of L-alanine and D-alanine to the enzyme | Lysinibacillus sphaericus | |
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] | Schiff base formation between D- or L-alanine and pyridoxal-5'-phophate in the active site | Lysinibacillus sphaericus | |
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] | binding of pimeloyl-CoA causes a conformational change in the active site | Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-carboxyhexanoyl-CoA + L-alanine | i.e. pimeloyl-CoA | Lysinibacillus sphaericus | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ir | |
6-carboxyhexanoyl-CoA + L-alanine | second step in biotin biosynthesis | Lysinibacillus sphaericus | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ir | |
6-carboxyhexanoyl-CoA + L-alanine | reaction in biotin biosynthesis | Lysinibacillus sphaericus | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ir |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Lysinibacillus sphaericus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | Schiff base formation between D- or L-alanine and pyridoxal-5'-phophate in the active site | Lysinibacillus sphaericus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
8-amino-7-oxo-8-phosphonononanoic acid | - |
Lysinibacillus sphaericus | |
0.068 | - |
4-carboxybutyl(1-amino-1-carboxyethyl)phosphonate | - |
Lysinibacillus sphaericus | |
0.08 | - |
2-amino-3-hydroxy-2-methylnonadioic acid | - |
Lysinibacillus sphaericus | |
0.59 | - |
D-alanine | - |
Lysinibacillus sphaericus |