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Literature summary for 2.3.1.47 extracted from

  • Izumi, Y.; Morita, H.; Tani, Y.; Ogata, K.
    Partial purification and some properties of a 7-keto-8-aminopelargonic acid synthetase, an enzyme involved in biotin biosynthesis (1973), Agric. Biol. Chem., 37, 1327-1333.
No PubMed abstract available
Search for more literature for 2.3.1.47

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline to some extent Lysinibacillus sphaericus
Ag+
-
 Lysinibacillus sphaericus
Cd2+ weak Lysinibacillus sphaericus
citrate to some extent Lysinibacillus sphaericus
Co2+
-
 Lysinibacillus sphaericus
Cu2+
-
 Lysinibacillus sphaericus
D-alanine
-
 Lysinibacillus sphaericus
D-cycloserine
-
 Lysinibacillus sphaericus
D-penicillamine L-enantiomer not as effective Lysinibacillus sphaericus
D/L-histidine
-
 Lysinibacillus sphaericus
diisopropyl fluorophosphate to some extent Lysinibacillus sphaericus
glycine
-
 Lysinibacillus sphaericus
hydroxylamine
-
 Lysinibacillus sphaericus
isoniazide to some extent Lysinibacillus sphaericus
L-cysteine strong Lysinibacillus sphaericus
L-serine
-
 Lysinibacillus sphaericus
additional information no inhibition by HgCl2, EDTA, 2,2'-dipyridyl, oxalate, KCN, various amino acids Lysinibacillus sphaericus
Ni2+
-
 Lysinibacillus sphaericus
phenylhydrazine
-
 Lysinibacillus sphaericus
Semicarbazide
-
 Lysinibacillus sphaericus
Zn2+ weak Lysinibacillus sphaericus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal ion requirement Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-carboxyhexanoyl-CoA + L-alanine Lysinibacillus sphaericus i.e. pimeloyl-CoA 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
6-carboxyhexanoyl-CoA + L-alanine Lysinibacillus sphaericus reaction in biotin biosynthesis 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Lysinibacillus sphaericus
recombinant from E. coli overproducing strain Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00225
-
 partially purified enzyme Lysinibacillus sphaericus

Storage Stability

Storage Stability Organism
-20°C, 0.01 M phosphate buffer, pH 7.0, 5 mM 2-mercaptoethanol, 5 months Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-carboxyhexanoyl-CoA + L-alanine absolutely specific for L-alanine Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
6-carboxyhexanoyl-CoA + L-alanine i.e. pimeloyl-CoA Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
6-carboxyhexanoyl-CoA + L-alanine reaction in biotin biosynthesis Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?

Synonyms

Synonyms Comment Organism
KAPA synthetase
-
 Lysinibacillus sphaericus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Lysinibacillus sphaericus
60
-
-
 Lysinibacillus sphaericus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 65 about half-maximal activity at 45°C and 65°C Lysinibacillus sphaericus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 and below, stable Lysinibacillus sphaericus
70
-
 t1/2: 10 min Lysinibacillus sphaericus
80
-
 and above, inactivation Lysinibacillus sphaericus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Lysinibacillus sphaericus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.5 about half-maximal activity at pH 6.5 and pH 7.5 Lysinibacillus sphaericus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate requirement, pyridoxal-5'-phosphate protein, not replaceable by pyridoxal, pyridoxine, pyridoxine 5-phosphate, pyridoxamine, pyridoxamine 5-phosphate Lysinibacillus sphaericus