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Literature summary for 2.3.1.47 extracted from

  • Ploux, O.; Marquet, A.
    The 8-amino-7-oxopelargonate synthase from Bacillus sphaericus. Purification and preliminary characterization of the cloned enzyme overproduced in Escherichia coli (1992), Biochem. J., 283, 327-331.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in overproducing Escherichia coli strain C268/pTG3401 Lysinibacillus sphaericus

General Stability

General Stability Organism
EDTA stabilizes during purification Lysinibacillus sphaericus
freeze-thawing inactivates Lysinibacillus sphaericus
proteinase inhibitors, e.g. PMSF, pepstatin A, stabilize during purification Lysinibacillus sphaericus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
pimeloyl-CoA
-
Lysinibacillus sphaericus
3
-
L-alanine
-
Lysinibacillus sphaericus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
amino acid composition Lysinibacillus sphaericus
41000
-
1 * 41000, SDS-PAGE Lysinibacillus sphaericus
46000
-
gel filtration Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-carboxyhexanoyl-CoA + L-alanine Lysinibacillus sphaericus i.e. pimeloyl-CoA 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ir
6-carboxyhexanoyl-CoA + L-alanine Lysinibacillus sphaericus reaction in biotin biosynthesis 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ir

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus
-
gene bioF
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from E. coli overproducing strain Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.67
-
-
Lysinibacillus sphaericus

Storage Stability

Storage Stability Organism
0°C, several months Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-carboxyhexanoyl-CoA + L-alanine i.e. pimeloyl-CoA Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ir
6-carboxyhexanoyl-CoA + L-alanine reaction in biotin biosynthesis Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ir

Subunits

Subunits Comment Organism
monomer 1 * 41000, SDS-PAGE Lysinibacillus sphaericus

Synonyms

Synonyms Comment Organism
AOP synthase
-
Lysinibacillus sphaericus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Lysinibacillus sphaericus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
pimeloyl-CoA
-
Lysinibacillus sphaericus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pI: 5.2 Lysinibacillus sphaericus
7
-
assay at Lysinibacillus sphaericus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Lysinibacillus sphaericus