Cloned (Comment) | Organism |
---|---|
PCR-amplification, transfection of Leishmania by electroporation | Leishmania major |
Protein Variants | Comment | Organism |
---|---|---|
additional information | null mutant deltalmdat/deltalmdat, and complemented null mutant, double mutants with additional loss of glycerol-3-phosphate acyltransferase (LmGAT) are not viable, thus, these two may be the only acyltransferases | Leishmania major |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glycosome | - |
Leishmania major | 20015 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone phosphate + acyl-CoA | Leishmania major | - |
1-acyl-glycerone phosphate + CoA | - |
? | |
dihydroxyacetone phosphate + acyl-CoA | Leishmania major V1 | - |
1-acyl-glycerone phosphate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | - |
V1 | - |
Leishmania major V1 | - |
V1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone phosphate + acyl-CoA | - |
Leishmania major | 1-acyl-glycerone phosphate + CoA | - |
? | |
dihydroxyacetone phosphate + acyl-CoA | - |
Leishmania major V1 | 1-acyl-glycerone phosphate + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHAPAT | - |
Leishmania major |
dihydroxyacetonephosphate acyltransferase | - |
Leishmania major |
LmDAT | - |
Leishmania major |
General Information | Comment | Organism |
---|---|---|
malfunction | null mutant produces longer lipophosphoglycan molecules, that migrate slower into the membrane and are not released into the medium, levels of glycosylphosphatidylinositol-anchored proteins are increased in the membrane (maybe due to slower trnasperot through the secretory pathway), however, the integrity of detergent resistant membranes is not affected, and the typical metacyclic genes such as SHERP are still expressed, arabinosylated forms of lipophosphoglycan are still produced, and the normal morphology is still exhibited | Leishmania major |
metabolism | catalyses initial step for glycerolipid metabolism, such as ether lipid derived virulence factor lipophosphoglycan and glycosylphosphatidylinositol-anchored proteins | Leishmania major |
physiological function | important for normal growth, survival during stationary phase, and virulence | Leishmania major |