Any feedback?
Please rate this page

BRENDA support

Literature summary for extracted from

  • Webber, K.O.; Hajra, A.K.
    Dihydroxyacetone phosphate acyltransferase (1992), Methods Enzymol., 209, 92-98.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
asolectin activation, i.e. crude soybean phospholipid preparation containing phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol Cavia porcellus
bovine serum albumin activation, by inhibiting the formation of palmitoyl-CoA micelles Cavia porcellus
additional information heating membranes to 50°C prior to assay increases activity of membrane-bound enzyme Cavia porcellus
Cavia porcellus

General Stability

General Stability Organism
heating membranes to 50°C prior to assay increases activity of membrane-bound enzyme Cavia porcellus


Inhibitors Comment Organism Structure
5,5'-dithio-bis(2-nitrobenzoate) 5 mM, moderate; i.e. DTNB Cavia porcellus
iodoacetamide not Cavia porcellus
N-ethylmaleimide not peroxisomal enzyme Cavia porcellus
p-chloromercuriphenylsulfonic acid moderate Cavia porcellus
palmitoyl-CoA bovine serum albumin protects Cavia porcellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
additional information
Cavia porcellus
0.04 0.2 dihydroxyacetone phosphate membrane-bound enzyme, pH 7.4 Cavia porcellus
dihydroxyacetone phosphate purified enzyme, pH 7.4 Cavia porcellus
0.1 0.5 dihydroxyacetone phosphate solubilized, pH 7.4 Cavia porcellus


Localization Comment Organism GeneOntology No. Textmining
Cavia porcellus 5777

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
x * 69000, SDS-PAGE Cavia porcellus
gel filtration Cavia porcellus


Organism UniProt Comment Textmining
Cavia porcellus

Purification (Commentary)

Purification (Comment) Organism
Cavia porcellus

Source Tissue

Source Tissue Comment Organism Textmining
Cavia porcellus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
purified enzyme Cavia porcellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + dihydroxyacetone phosphate
Cavia porcellus CoA + acyldihydroxyacetone phosphate i.e. acylglycerone phosphate ?
palmitoyl-CoA + dihydroxyacetone phosphate
Cavia porcellus CoA + monopalmitoyldihydroxyacetone phosphate


Subunits Comment Organism
? x * 69000, SDS-PAGE Cavia porcellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
assay at Cavia porcellus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
membrane-bound: heat-stable Cavia porcellus
additional information
solubilized: heat-labile Cavia porcellus
significant loss of activity Cavia porcellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
membrane-bound peroxisomal enzyme Cavia porcellus
detergent-solubilized enzyme Cavia porcellus