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Literature summary for 2.3.1.159 extracted from

  • Lukacin.R.; Springob, K.; Urbanke, C.; Ernwein, C.; Schröder, G.; Schröder, J.; Matern, U.
    Native acridone synthases I and II from Ruta graveolens L. form homodimers (1999), FEBS Lett., 448, 135-140.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain M15 Ruta graveolens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
malonyl-CoA isozyme II Ruta graveolens
0.013
-
malonyl-CoA isozyme I Ruta graveolens
0.062
-
N-methylanthraniloyl-CoA isozyme I Ruta graveolens
0.077
-
N-methylanthraniloyl-CoA isozyme II Ruta graveolens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
isozyme I, gel filtration Ruta graveolens
45000
-
about, isozyme II, gel filtration Ruta graveolens
81000
-
isozyme I, analytical ultracentrifugation Ruta graveolens
82000
-
isozyme II, analytical ultracentrifugation Ruta graveolens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonyl-CoA + N-methylanthraniloyl-CoA Ruta graveolens involved in the biosynthesis of all acridone alkaloids CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
?

Organism

Organism UniProt Comment Textmining
Ruta graveolens
-
2 isozymes I and II
-

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture strain R-20 Ruta graveolens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.18
-
crude extract Ruta graveolens
9.54
-
purified recombinant isozyme I Ruta graveolens
14.16
-
purified recombinant isozyme II Ruta graveolens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + N-methylanthraniloyl-CoA
-
Ruta graveolens CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
?
malonyl-CoA + N-methylanthraniloyl-CoA involved in the biosynthesis of all acridone alkaloids Ruta graveolens CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 44000-45000, isozyme I and II, analytical ultracentrifugation and SDS-PAGE Ruta graveolens

Synonyms

Synonyms Comment Organism
ACS
-
Ruta graveolens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
isozyme I Ruta graveolens
40
-
isozyme II Ruta graveolens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
isozyme II Ruta graveolens
7.5
-
isozyme I Ruta graveolens

pH Range

pH Minimum pH Maximum Comment Organism
6 9 isozyme I, assay range Ruta graveolens
6 7.5 isozyme II, assay range Ruta graveolens