Cloned (Comment) | Organism |
---|---|
AAT1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree of BAHD acyltransferases, expression of GST-tagged AAT1 in Escherichia coli strain Rosetta(DE3)pLysS | Lavandula angustifolia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
4-coumaroyl-CoA | with 3-(4-hydroxyphenyl)lactate, pH 7.5, 30°C, recombinant AAT1 | Lavandula angustifolia | |
0.021 | - |
3-(4-hydroxyphenyl)lactate | with 4-coumaroyl-CoA, pH 7.5, 30°C, recombinant AAT1 | Lavandula angustifolia | |
0.022 | - |
feruloyl-CoA | with 3-(4-hydroxyphenyl)lactate, pH 7.5, 30°C, recombinant AAT1 | Lavandula angustifolia | |
0.025 | - |
cinnamoyl-CoA | with 3-(4-hydroxyphenyl)lactate, pH 7.5, 30°C, recombinant AAT1 | Lavandula angustifolia | |
0.049 | - |
3-(4-hydroxyphenyl)lactate | with caffeoyl-CoA, pH 7.5, 30°C, recombinant AAT1 | Lavandula angustifolia | |
0.05 | - |
caffeoyl-CoA | with 3-(4-hydroxyphenyl)lactate, pH 7.5, 30°C, recombinant AAT1 | Lavandula angustifolia | |
0.083 | - |
4-coumaroyl-CoA | with shikimate, pH 7.5, 30°C, recombinant AAT1 | Lavandula angustifolia | |
0.652 | - |
3-(4-hydroxyphenyl)lactate | with cinnamoyl-CoA, pH 7.5, 30°C, recombinant AAT1 | Lavandula angustifolia | |
0.672 | - |
3-(4-hydroxyphenyl)lactate | with feruloyl-CoA, pH 7.5, 30°C, recombinant AAT1 | Lavandula angustifolia |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Lavandula angustifolia | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50600 | - |
1 * 50600, about, LAAT1, sequence determination | Lavandula angustifolia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
caffeoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate | Lavandula angustifolia | - |
CoA + rosmarinate | - |
? | |
additional information | Lavandula angustifolia | recombinant LaAAT1 exhibits a broad substrate tolerance accepting (hydroxy)cinnamoyl-CoAs as acyl donors and not only tyramine, tryptamine, phenylethylamine, and anthranilic acid but also shikimic acid and 4-hydroxyphenyllactic acid as acceptors, showing substrate promiscuity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lavandula angustifolia | Q0GA14 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged AAT1 from Escherichia coli by cell disruption through freeze-thaw cycles, and glutathione affinity chromatography | Lavandula angustifolia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Lavandula angustifolia | - |
Storage Stability | Organism |
---|---|
4°C, purified recombinant GST-tagged AAT1, in 50 mM Tris-Cl, pH 8.0, and 10 mM reduced glutathione, 4 weeks, stable | Lavandula angustifolia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + 3-(4-hydroxyphenyl)lactate | - |
Lavandula angustifolia | CoA + 2-O-(4-coumaroyl)-3-(4-hydroxyphenyl)lactate | - |
? | |
4-coumaroyl-CoA + shikimate | - |
Lavandula angustifolia | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
caffeoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate | - |
Lavandula angustifolia | CoA + rosmarinate | - |
? | |
caffeoyl-CoA + 3-(4-hydroxyphenyl)lactate | - |
Lavandula angustifolia | CoA + 2-O-caffeoyl-3-(4-hydroxyphenyl)lactate | - |
? | |
cinnamoyl-CoA + 3-(4-hydroxyphenyl)lactate | - |
Lavandula angustifolia | CoA + 2-O-cinnamoyl-3-(4-hydroxyphenyl)lactate | - |
? | |
feruloyl-CoA + 3-(4-hydroxyphenyl)lactate | - |
Lavandula angustifolia | CoA + 2-O-feruloyl-3-(4-hydroxyphenyl)lactate | - |
? | |
additional information | recombinant LaAAT1 exhibits a broad substrate tolerance accepting (hydroxy)cinnamoyl-CoAs as acyl donors and not only tyramine, tryptamine, phenylethylamine, and anthranilic acid but also shikimic acid and 4-hydroxyphenyllactic acid as acceptors, showing substrate promiscuity | Lavandula angustifolia | ? | - |
? | |
additional information | substrate specificity of recombinant AAT1, overview. No or poor activity with anthranilic acid | Lavandula angustifolia | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50600, about, LAAT1, sequence determination | Lavandula angustifolia |
Synonyms | Comment | Organism |
---|---|---|
AAT1 | - |
Lavandula angustifolia |
AT1 | - |
Lavandula angustifolia |
rosmarinic acid synthase | - |
Lavandula angustifolia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Lavandula angustifolia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Lavandula angustifolia |
General Information | Comment | Organism |
---|---|---|
evolution | rosmarinic acid synthase is a member of the plant BAHD family, it contains the BAHD family HXXXD motif (amino acids 180-184), with the catalytically active histidine and the DFGWG motif that is thought to be responsible for the steric position of the active site, but it additionally contains 25 additional amino acids in the section following the HXXXD motif, phylogenetic tree of BAHD acyltransferases, overview | Lavandula angustifolia |