Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Lactobacillus acidophilus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 2 M ammonium sulfate, 0.2M potassium sodium tartrate tetrahydrate and 0.1 M sodium citrate tribasic dehydrate, pH 5.6 | Lactobacillus acidophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.092 | - |
S-adenosyl-L-methionine | with Tris/NaHCO3, at pH 8.0 and 37°C | Lactobacillus acidophilus | |
0.113 | - |
S-adenosyl-L-methionine | with Tris, at pH 8.0 and 37°C | Lactobacillus acidophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + phospholipid olefinic fatty acid | Lactobacillus acidophilus | - |
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid | - |
? | |
S-adenosyl-L-methionine + phospholipid olefinic fatty acid | Lactobacillus acidophilus NCFM | - |
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus acidophilus | Q5FJM0 | - |
- |
Lactobacillus acidophilus NCFM | Q5FJM0 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex S200 gel filtration | Lactobacillus acidophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + phospholipid olefinic fatty acid | - |
Lactobacillus acidophilus | S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid | - |
? | |
S-adenosyl-L-methionine + phospholipid olefinic fatty acid | - |
Lactobacillus acidophilus NCFM | S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE | Lactobacillus acidophilus |
Synonyms | Comment | Organism |
---|---|---|
CFA synthase | - |
Lactobacillus acidophilus |
CFAS | - |
Lactobacillus acidophilus |
cyclopropane-fatty-acyl-phospholipid synthase | - |
Lactobacillus acidophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
S-adenosyl-L-methionine | with Tris, at pH 8.0 and 37°C | Lactobacillus acidophilus | |
0.071 | - |
S-adenosyl-L-methionine | with Tris/NaHCO3, at pH 8.0 and 37°C | Lactobacillus acidophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
S-adenosyl-L-methionine | with Tris, at pH 8.0 and 37°C | Lactobacillus acidophilus | |
0.77 | - |
S-adenosyl-L-methionine | with Tris/NaHCO3, at pH 8.0 and 37°C | Lactobacillus acidophilus |