Protein Variants | Comment | Organism |
---|---|---|
K139A | site-directed mutagenesis, 2-3fold reduced DNA binding compared to the wild-type enzyme | Escherichia coli |
L122A | site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme, DNA binding is similar to the wild-type enzyme | Escherichia coli |
N120A | site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme, DNA binding is similar to the wild-type enzyme | Escherichia coli |
N120S | site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme, DNA binding is similar to the wild-type enzyme | Escherichia coli |
P134A | site-directed mutagenesis, 2-3fold reduced DNA binding compared to the wild-type enzyme | Escherichia coli |
P134G | site-directed mutagenesis, 2-3fold reduced DNA binding compared to the wild-type enzyme | Escherichia coli |
R124A | site-directed mutagenesis, over 100fold reduced activity and about 10fold reduced DNA binding compared to the wild-type enzyme | Escherichia coli |
R137A | site-directed mutagenesis, DNA binding is similar to the wild-type enzyme | Escherichia coli |
Y119A | site-directed mutagenesis, over 100fold reduced activity compared to the wild-type enzyme, 2-3fold reduced DNA binding compared to the wild-type enzyme | Escherichia coli |
Y138A | site-directed mutagenesis, DNA binding is similar to the wild-type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sinefungin | i.e. adenosyl ornithine, interaction mechanism with the enzyme, binding site structure, stabilization of the flipped adenine in presence of the inhibitor | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | Escherichia coli | - |
S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | Tequatrovirus T4 | specific target sequence is GATC | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Tequatrovirus T4 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | DNA substrate specificities of wild-type and mutant enzymes | Tequatrovirus T4 | ? | - |
? | |
S-adenosyl-L-methionine + DNA adenine | - |
Escherichia coli | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | specific target sequence is GATC | Tequatrovirus T4 | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | specific target sequence is GATC, transition of enzyme-DNA interaction from nonspecific to specific interaction utilizing different substrates, identification of discriminatory contacts stabilizing the transition state, and antidiscriminatory contacts not affecting the methylation of the cognate site but disfavor activity at noncognate sites, involved residues are M114, R116, P126, G128, R130, F111, S112, D171, K11, and Y174, overview, flipping of target adenine | Tequatrovirus T4 | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | transition of enzyme-DNA interaction from nonspecific to specific interaction utilizing different substrates, identification of discriminatory contacts stabilizing the transition state, and antidiscriminatory contacts not affecting the methylation of the cognate site but disfavor activity at noncognate sites, involved are Y119, N120, L122, R124, and P134, overview | Escherichia coli | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure analysis of enzyme in ternary complex with partially and fully specific DNA and a methyl-donor analogue | Escherichia coli |
More | structure analysis of enzyme in ternary complex with partially and fully specific DNA and S-adenosyl-L-homocysteine, S-adenosyl-L-methionine, or inhibitor adenosyl ornithine, i.e. sinefungin | Tequatrovirus T4 |
Synonyms | Comment | Organism |
---|---|---|
DNA-adenine methyltransferase | - |
Escherichia coli |
EcoDam | - |
Escherichia coli |
T4 DNA-adenine methyltransferase | - |
Tequatrovirus T4 |
T4Dam | - |
Tequatrovirus T4 |