Activating Compound | Comment | Organism | Structure |
---|---|---|---|
TRM112 | Trm112 is a small zinc finger protein of 15 kDa which acts as a coactivator of several class I S-adenosyl-L-methionine (SAM)-dependent MTases. Trm112 interacts directly with Bud23 in vitro and that it is absolutely required for Bud23 stability in vivo. Trm112 increases the solubility of Bud23 | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
recombinant co-expression of His6-tagged Bud23 with untagged Trm112 in Escherichia coli | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
Trm112 interacts directly with Bud23 in vitro and that it is required for Bud23 stability in vivo | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | Trm112 interacts directly with Bud23 in vitro and that it is required for Bud23 stability in vivo | ? | - |
? | |
S-adenosyl-L-methionine + guanine1575 in 18S rRNA | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + N7-methylguanine1575 in 18S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
tandem purification of recombinant His6-tagged Bud23 with untagged Trm112 by nickel affinity chromatography from Escherichia coli, Trm112 increases the solubility of Bud23 | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Trm112 interacts directly with Bud23 in vitro and that it is required for Bud23 stability in vivo | Saccharomyces cerevisiae | ? | - |
? | |
S-adenosyl-L-methionine + guanine1575 in 18S rRNA | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N7-methylguanine1575 in 18S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
18S rRNA methyltransferase | - |
Saccharomyces cerevisiae |
BUD23 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
evolution | Bud23 is a class I S-adenosyl-L-methionine (SAM)-dependent MTase | Saccharomyces cerevisiae |
malfunction | trm112DELTA cells are deficient for Bud23-mediated 18S rRNA methylation at position G1575 and for small ribosome subunit formation. Bud23 failure to bind nascent preribosomes activates a nucleolar surveillance pathway involving the TRAMP complexes, leading to preribosome degradation | Saccharomyces cerevisiae |
metabolism | Bud23 failure to bind nascent preribosomes activates a nucleolar surveillance pathway involving the TRAMP complexes, leading to preribosome degradation | Saccharomyces cerevisiae |