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Literature summary for 2.1.1.299 extracted from
- The N-terminal methyltransferase homologs NRMT1 and NRMT2 exhibit novel regulation of activity through heterotrimer formation (2018), Protein Sci., 27, 1585-1599 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene NTMT2, recombinant expression of His-tagged NRMT2 in Escherichia coli and of wild-type and mutant FLAG-tagged NRMT2s in HEK-293 cells, recombinant expression of GFP-tagged enzyme in HCT-116 cells | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| analysis of crystal structures of NRMT1 and NRMT2 (PDB IDs 2EX4 and 5UBB, determined to 1.75 and 2.0 A, respectively), homology modeling | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | co-expression of NRMT2 with NRMT1 increases the trimethylation rate of NRMT1. Generation of truncation constructs of NRMT2 | Homo sapiens |
| V224L | site-directed mutagenesis, inactive mutant | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0556 | - |
SPKRIAKRRSPPADA | recombinant NRMT2, pH and temperature not specified in the publication | Homo sapiens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Homo sapiens | 5634 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35700 | - |
monomeric recombinant enzyme, analytical ultracentrifugation | Homo sapiens |
| 52600 | - |
dimeric recombinant enzyme, analytical ultracentrifugation | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + N-terminal-(A,P,S)PK-[protein] | Homo sapiens | - |
S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-(A,P,S)PK-[protein] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q5VVY1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged NRMT2 from Escherichia coli | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| colorectal cancer cell | - |
Homo sapiens | - |
| HCT-116 cell | - |
Homo sapiens | - |
| additional information | NRMT2 is a monomethylase expressed at low levels in a tissue-specific manner | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | NRMT2 exhibits monomethylase activity in vitro. Isozymes NRMT1 and NRMT2 can interact both in vitro and in vivo, modeling of NRMT1 and NRMT2 interactions. No activity with the MYL9 peptide SSKRAKAKTTKKRP | Homo sapiens | ? | - |
- |
|
| S-adenosyl-L-methionine + N-terminal-(A,P,S)PK-[protein] | - |
Homo sapiens | S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-(A,P,S)PK-[protein] | - |
? | |
| S-adenosyl-L-methionine + SPKRIAKRRSPPADA | substrate peptide consisting of the first 15 amino acids of RCC1 | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 35700, recombinant isozyme NRMT2, SDS-PAGE and analytical ultracentrifugation | Homo sapiens |
| More | enzyme NRMT1 primarily exists as a monomer. Isozymes NRMT1 and NRMT2, when co-expressed, form a heterotrimer, interaction analysis, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha N-terminal protein methyltransferase 1B | UniProt | Homo sapiens |
| METTL11B | gene name, UniProt | Homo sapiens |
| N-terminal RCC1 methyltransferase 2 | - |
Homo sapiens |
| NRMT2 | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the methyltransferase like (METTL) family of class I methyltransferases containing seven-beta-strand methyltransferase motifs and Rossman folds for binding SAM. The N-terminal methyltransferase homologs NRMT1 (N-terminal RCC1 methyltransferase 1) and NRMT2 (N-terminal RCC1 methyltransferase 2), which following cleavage of the initiating methionine, methylate the alpha-amine of the first N-terminal residue of their substrates. NRMT1 and NRMT2 are 50% identical and 75% similar and share an N-terminal X-P-K consensus sequence. Although structurally similar, they differ in their catalytic activities | Homo sapiens |
| additional information | analysis of crystal structures of NRMT1 and NRMT2 (PDB IDs 2EX4 and 5UBB, determined to 1.75 and 2.0 A, respectively), homology modeling. Modeling of NRMT1 and NRMT2 heterotrimer, interaction analysis, overview | Homo sapiens |
| physiological function | isozyme NRMT2 expression activates isozyme NRMT1 activity, not through priming, but by increasing its stability and substrate affinity. NRMT2 overexpression cannot rescue NRMT1 knockout phenotypes | Homo sapiens |
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