Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Astragalus bisulcatus |
cloned into plasmid pT7-7 and overexpression in Escherichia coli BL21(DE3) cells | Escherichia coli |
pUKE vector used for cloning of cDNA sequence coding for the SeCys-methyltransferase from Astragalus bisulcatus in Escherichia coli | Astragalus bisulcatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-methionine | product inhibition above 0.5 mM | Escherichia coli | |
S-adenosyl-L-methionine | product inhibition above 0.25 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
DL-selenohomocysteine | pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine | Escherichia coli | |
0.043 | - |
DL-selenohomocysteine | app. Km-value with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C | Escherichia coli | |
0.045 | - |
L-homocysteine | app. Km-value with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C | Escherichia coli | |
0.35 | - |
DL-selenohomocysteine | pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine | Astragalus bisulcatus | |
0.35 | - |
DL-selenohomocysteine | app. Km-value with [methyl-14C]S-adenosyl-L-methionine, pH 6.0, 30°C | Astragalus bisulcatus | |
0.59 | - |
L-selenocysteine | pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine | Escherichia coli | |
0.59 | - |
L-selenocysteine | app. Km-valuen with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C | Escherichia coli | |
0.7 | - |
L-selenocysteine | pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine | Astragalus bisulcatus | |
0.7 | - |
L-selenocysteine | app. Km-value with [methyl-14C]S-adenosyl-L-methionine, pH 6.0, 30°C | Astragalus bisulcatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Astragalus bisulcatus | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22500 | - |
1 * 22500, calculated from sequence | Escherichia coli |
33500 | - |
calculated from gene sequence | Escherichia coli |
34000 | - |
gel filtration | Escherichia coli |
35000 | - |
SDS-PAGE | Escherichia coli |
36700 | - |
calculated from nucleotide sequence | Astragalus bisulcatus |
36700 | - |
x * 36700, calculated from sequence | Astragalus bisulcatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-methyl-L-methionine + L-selenocysteine | Escherichia coli | - |
L-methionine + Se-methyl-L-selenocysteine | - |
? | |
S-methyl-L-methionine + L-selenocysteine | Escherichia coli | S-methylmethionine is the physiological methyl group donor | L-methionine + Se-methyl-L-selenocysteine | - |
? | |
S-methyl-L-methionine + L-selenocysteine | Astragalus bisulcatus | S-methylmethionine is the physiological methyl group donor. The enzyme is involved in detoxification of selenium in selenium-accumulating plants | L-methionine + Se-methyl-L-selenocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Astragalus bisulcatus | - |
- |
- |
Astragalus bisulcatus | P56707 | - |
- |
Escherichia coli | Q47690 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Astragalus bisulcatus |
by ammonium sulfate precipitation, hydrophobic interaction chromatography and anion exchange chromatography with Q-Sepharose | Astragalus bisulcatus |
by ammonium sulfate precipitation, hydrophobic interaction chromatography and anion exchange chromatography with Q-Sepharose | Escherichia coli |
recombinant enzyme | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | cell cultures contain the maximal level of selenocysteine methyltransferase under conditions of exponential growth. Stationary phase cells possess a much lower amount | Astragalus bisulcatus | - |
cotyledon | of 4-8-week-old seedlings | Astragalus bisulcatus | - |
cotyledon | immunoblotting analysis | Astragalus bisulcatus | - |
hypocotyl | of 4-8-week-old seedlings | Astragalus bisulcatus | - |
hypocotyl | immunoblotting analysis | Astragalus bisulcatus | - |
leaf | primary of 4-8-week-old seedlings | Astragalus bisulcatus | - |
primary leaf | immunoblotting analysis | Astragalus bisulcatus | - |
root | of 4-8-week-old seedlings | Astragalus bisulcatus | - |
root | immunoblotting analysis | Astragalus bisulcatus | - |
seedling | - |
Astragalus bisulcatus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
with L-S-methylmethionine, maximal conversion is 100%, pH 6.0, 30°C | Astragalus bisulcatus |
0.016 | - |
with [methyl-14C]S-adenosyl-L-methionine, maximal conversion is 10%, pH 6.0, 30°C | Astragalus bisulcatus |
0.68 | - |
with S-adenosyl-L-methionine, maximal conversion is 20-25%, pH 6.0, 30°C | Astragalus bisulcatus |
1.5 | - |
with DL-S-methylmethionine, maximal conversion is 50%, pH 6.0, 30°C | Astragalus bisulcatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme also catalyzes the reaction of EC 2.1.1.10 | Escherichia coli | ? | - |
? | |
additional information | no activity with trimethylsulfonium and glycine betaine as methyl donor | Astragalus bisulcatus | ? | - |
? | |
additional information | no activity with trimethylsulfonium and glycine betaine as methyl donor | Escherichia coli | ? | - |
? | |
S-adenosyl-L-methionine + 4-thiobutyric acid | 0.014% relative activity compared to activity with L-homocysteine set at 100% | Escherichia coli | S-adenosyl-L-homocysteine + 4-methylthiobutyric acid | - |
? | |
S-adenosyl-L-methionine + D-homocysteine | 16% relative activity compared to activity with L-homocysteine set at 100% | Escherichia coli | ? | - |
? | |
S-adenosyl-L-methionine + DL-homocysteine | 93% relative activity compared to activity with L-homocysteine set at 100% | Escherichia coli | ? | - |
? | |
S-adenosyl-L-methionine + DL-selenohomocysteine | 213% relative activity compared to activity with L-homocysteine set at 100% | Escherichia coli | ? | - |
? | |
S-adenosyl-L-methionine + L-cysteine | less than 0.003% relative activity compared to activity with L-homocysteine set at 100% | Escherichia coli | S-adenosyl-L-homocysteine + S-methyl-L-cysteine | - |
? | |
S-adenosyl-L-methionine + L-homocysteine | - |
Escherichia coli | ? | - |
? | |
S-adenosyl-L-methionine + L-homoserine | less than 0.003% relative activity compared to activity with L-homocysteine set at 100% | Escherichia coli | ? | - |
? | |
S-adenosyl-L-methionine + L-selenocysteine | - |
Astragalus bisulcatus | S-adenosyl-L-homocysteine + Se-methyl-L-selenocysteine | - |
? | |
S-adenosyl-L-methionine + L-selenocysteine | - |
Escherichia coli | S-adenosyl-L-homocysteine + Se-methyl-L-selenocysteine | - |
? | |
S-adenosyl-L-methionine + L-selenocysteine | S-adenosyl-L-methionine at intracellular concentrations cannot serve as an effective substrate for the enzyme | Astragalus bisulcatus | S-adenosyl-L-homocysteine + Se-methyl-L-selenocysteine | - |
? | |
S-adenosyl-L-methionine + L-selenocysteine | 0.027% relative activity compared to activity with L-homocysteine set at 100% | Escherichia coli | S-adenosyl-L-homocysteine + Se-methyl-L-selenocysteine | - |
? | |
S-adenosyl-L-methionine + L-selenohomocysteine | - |
Escherichia coli | ? | - |
? | |
S-methyl-L-methionine + DL-selenohomocysteine | - |
Astragalus bisulcatus | ? | - |
? | |
S-methyl-L-methionine + L-selenocysteine | - |
Astragalus bisulcatus | L-methionine + Se-methyl-L-selenocysteine | - |
? | |
S-methyl-L-methionine + L-selenocysteine | - |
Escherichia coli | L-methionine + Se-methyl-L-selenocysteine | - |
? | |
S-methyl-L-methionine + L-selenocysteine | S-methylmethionine is the physiological methyl group donor | Escherichia coli | L-methionine + Se-methyl-L-selenocysteine | - |
? | |
S-methyl-L-methionine + L-selenocysteine | S-methylmethionine is the physiological methyl group donor. The enzyme is involved in detoxification of selenium in selenium-accumulating plants | Astragalus bisulcatus | L-methionine + Se-methyl-L-selenocysteine | - |
? | |
S-methyl-L-methionine + L-selenocysteine | activity with S-methyl-L-methionine is higher than with S-adenosyl-L-methionine | Escherichia coli | L-methionine + Se-methyl-L-selenocysteine | - |
? | |
S-methyl-L-methionine + L-selenocysteine | activity with S-methyl-L-methionine is higher than with S-adenosyl-L-methionine | Astragalus bisulcatus | L-methionine + Se-methyl-L-selenocysteine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36700, calculated from sequence | Astragalus bisulcatus |
monomer | 1 * 35000, SDS-PAGE | Escherichia coli |
monomer | 1 * 22500, calculated from sequence | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
Hcy methyltransferase | - |
Escherichia coli |
SeCys-methyltransferase | - |
Astragalus bisulcatus |
SeCys-MT | - |
Astragalus bisulcatus |
selenocysteine methyltransferase | - |
Astragalus bisulcatus |
smtA | - |
Astragalus bisulcatus |
YagD | - |
Escherichia coli |
YagD protein | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00017 | - |
L-selenocysteine | pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine | Escherichia coli | |
0.00017 | - |
L-selenocysteine | with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C | Escherichia coli | |
0.0062 | - |
L-selenocysteine | pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine | Astragalus bisulcatus | |
0.0062 | - |
L-selenocysteine | with [methyl-14C]S-adenosyl-L-methionine, pH 6.0, 30°C | Astragalus bisulcatus | |
0.0074 | - |
DL-selenohomocysteine | pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine | Astragalus bisulcatus | |
0.0074 | - |
DL-selenohomocysteine | with [methyl-14C]S-adenosyl-L-methionine, pH 6.0, 30°C | Astragalus bisulcatus | |
0.38 | - |
L-homocysteine | with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C | Escherichia coli | |
0.74 | - |
DL-selenohomocysteine | pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine | Escherichia coli | |
0.74 | - |
DL-selenohomocysteine | with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | detoxification of selenium-containing compounds | Astragalus bisulcatus |
metabolism | detoxification of selenium-containing compounds | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00029 | - |
L-selenocysteine | with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C | Escherichia coli | |
0.0089 | - |
L-selenocysteine | with [methyl-14C]S-adenosyl-L-methionine, pH 6.0, 30°C | Astragalus bisulcatus | |
0.021 | - |
DL-selenohomocysteine | with [methyl-14C]S-adenosyl-L-methionine, pH 6.0, 30°C | Astragalus bisulcatus | |
8.4 | - |
L-homocysteine | with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C | Escherichia coli | |
17 | - |
DL-selenohomocysteine | with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C | Escherichia coli |