Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, expression in Arabidopsis thaliana under the control of the 35S cauliflower mosaic virus promoter | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 5 mM, more than 90% inhibition | Arabidopsis thaliana | |
Fe3+ | 5 mM, more than 90% inhibition | Arabidopsis thaliana | |
Zn2+ | 5 mM, more than 90% inhibition | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
gibberellin a9 | pH 8.0, 30°C | Arabidopsis thaliana | |
0.0059 | - |
gibberellin a4 | pH 8.0, 30°C | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not enhanced nor inhibited by K+, NH4+, Na+, Ca2+, Fe2þ, Mg2+, and Mn2+ | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43300 | - |
x * 43300, calculated from sequence | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + gibberellin A4 | Arabidopsis thaliana | - |
S-adenosyl-L-homocysteine + methyl gibberellin A4 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q5XF78 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Arabidopsis thaliana | - |
silique | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with gibberelin A12 and gibberelin A19 | Arabidopsis thaliana | ? | - |
? | |
S-adenosyl-L-methionine + gibberellin A1 | 30% of the activity compared to methylation of gibberellin A4 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A1 | - |
? | |
S-adenosyl-L-methionine + gibberellin A20 | 15% of the activity compared to methylation of gibberellin A4 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A20 | - |
? | |
S-adenosyl-L-methionine + gibberellin A3 | 45% of the activity compared to methylation of gibberellin A4 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A3 | - |
? | |
S-adenosyl-L-methionine + gibberellin A34 | 80% of the activity compared to methylation of gibberellin A4 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A34 | - |
? | |
S-adenosyl-L-methionine + gibberellin A4 | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A4 | - |
? | |
S-adenosyl-L-methionine + gibberellin A51 | 16% of the activity compared to methylation of gibberellin A4 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A51 | - |
? | |
S-adenosyl-L-methionine + gibberellin A9 | 60% of the activity compared to methylation of gibberellin A4 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A9 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43300, calculated from sequence | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
GAMT2 | - |
Arabidopsis thaliana |
gibberellin A4 O-methyltransferase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
gibberellin a4 | pH 8.0, 30°C | Arabidopsis thaliana | |
0.0018 | - |
gibberellin a9 | pH 8.0, 30°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | expressed mainly in whole siliques, peak transcript levels from the middle until the end of silique development | up |
General Information | Comment | Organism |
---|---|---|
malfunction | GAMT2 null mutant shows reduced inhibition of germination, compared with the wild type, when placed on plates containing the gibberellin biosynthesis inhibitor ancymidol | Arabidopsis thaliana |
physiological function | methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
gibberellin a4 | pH 8.0, 30°C | Arabidopsis thaliana | |
0.9 | - |
gibberellin a9 | pH 8.0, 30°C | Arabidopsis thaliana |