Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-AI cells | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0146 | - |
S-adenosyl-L-methionine | in 50 mM Tris-HCl, pH 7.5, 100 mM KCl, at 25°C | Solanum lycopersicum | |
0.05 | - |
salicylate | in 50 mM Tris-HCl, pH 7.5, 100 mM KCl, at 25°C | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Solanum lycopersicum | less than 1% activity with jasmonic acid, indole-3-acetic acid and gibberellic acid 3 | ? | - |
? | |
S-adenosyl-L-methionine + 4-aminosalicylate | Solanum lycopersicum | 2% activity compared to salicylate | S-adenosyl-L-homocysteine + methyl 4-aminosalicylate | - |
? | |
S-adenosyl-L-methionine + benzoate | Solanum lycopersicum | 2% activity compared to salicylate | S-adenosyl-L-homocysteine + methyl benzoate | - |
? | |
S-adenosyl-L-methionine + salicylate | Solanum lycopersicum | 100% activity | S-adenosyl-L-homocysteine + methyl salicylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bud | - |
Solanum lycopersicum | - |
flower | - |
Solanum lycopersicum | - |
fruit | - |
Solanum lycopersicum | - |
leaf | - |
Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | less than 1% activity with jasmonic acid, indole-3-acetic acid and gibberellic acid 3 | Solanum lycopersicum | ? | - |
? | |
S-adenosyl-L-methionine + 4-aminosalicylate | 2% activity compared to salicylate | Solanum lycopersicum | S-adenosyl-L-homocysteine + methyl 4-aminosalicylate | - |
? | |
S-adenosyl-L-methionine + benzoate | 2% activity compared to salicylate | Solanum lycopersicum | S-adenosyl-L-homocysteine + methyl benzoate | - |
? | |
S-adenosyl-L-methionine + salicylate | 100% activity | Solanum lycopersicum | S-adenosyl-L-homocysteine + methyl salicylate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
salicylic acid methyl transferase | - |
Solanum lycopersicum |
SAMT | - |
Solanum lycopersicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
S-adenosyl-L-methionine | in 50 mM Tris-HCl, pH 7.5, 100 mM KCl, at 25°C | Solanum lycopersicum | |
0.056 | - |
salicylate | in 50 mM Tris-HCl, pH 7.5, 100 mM KCl, at 25°C | Solanum lycopersicum |
Organism | Comment | Expression |
---|---|---|
Solanum lycopersicum | isoform SAMT1 expression drops off to essentially undetectable levels by the time fruits are fully ripened | down |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is critical for methyl salicylate synthesis | Solanum lycopersicum |