Activating Compound | Comment | Organism | Structure |
---|---|---|---|
queuine | PMT1 is strongly stimulated by prior queuosine (Q) modification of the substrate tRNA. In vivo tRNA methylation levels are stimulated by growth of cells in queuine-containing medium, in vitro Pmt1 activity is enhanced on Q-containing RNA; and queuine-stimulated in vivo methylation is abrogated by the absence of the eukaryotic tRNA-guanine transglycosylase | Schizosaccharomyces pombe | |
queuine | the addition of queuine to the growth medium increases C38 methylation from 10 to 100%, while other cytosines remain unaffected | Dictyostelium discoideum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | Q54JH6 | cf. EC 2.1.1.37 | - |
Schizosaccharomyces pombe | P40999 | cf. EC 2.1.1.204 | - |
Schizosaccharomyces pombe 972 | P40999 | cf. EC 2.1.1.204 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytosine34 in tRNAPro | - |
Schizosaccharomyces pombe | S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNAPro | - |
? | |
S-adenosyl-L-methionine + cytosine34 in tRNAPro | - |
Dictyostelium discoideum | S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNAPro | - |
? | |
S-adenosyl-L-methionine + cytosine34 in tRNAPro | - |
Schizosaccharomyces pombe 972 | S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNAPro | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DnmA | - |
Dictyostelium discoideum |
PMT1 | - |
Schizosaccharomyces pombe |
PMT1 | - |
Dictyostelium discoideum |