Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activity is increased, in both in vitro and in vivo carnation tissues, by the inoculation with Fusarium oxysporum f. sp.dianthi | Dianthus caryophyllus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, 19% inhibition | Dianthus caryophyllus | |
iodoacetamide | 1 mM, 60% inhibition | Dianthus caryophyllus | |
Mg2+ | 10 mM, 27% inhibition | Dianthus caryophyllus | |
Mn2+ | 1 mM: 21% inhibition, 10 mM: 57% inhibition | Dianthus caryophyllus | |
Phenylmercuriacetate | 1 mM, 80% inhibition | Dianthus caryophyllus | |
S-adenosyl-L-homocysteine | competitive, inhibition kinetics | Dianthus caryophyllus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic mechanism | Dianthus caryophyllus | |
0.0017 | - |
kaempferol | pH 7, 25°C | Dianthus caryophyllus | |
0.0033 | - |
apigenin | pH 7, 25°C | Dianthus caryophyllus | |
0.011 | - |
4'-hydroxyflavanone | pH 7, 25°C | Dianthus caryophyllus | |
0.0735 | - |
genistein | pH 7, 25°C | Dianthus caryophyllus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no divalent metal ion requirement | Dianthus caryophyllus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | 45000 | gel filtration, native PAGE | Dianthus caryophyllus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Dianthus caryophyllus | the role of F 4-OMT is mainly defensive against parasites, rather than structural | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dianthus caryophyllus | - |
cv. Novada | - |
Purification (Comment) | Organism |
---|---|
1399fold | Dianthus caryophyllus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + kaempferol = S-adenosyl-L-homocysteine + kaempferide | the enzyme acts on the hydroxy group in the 4'-position of some flavones, flavanones and isoflavones, kaempferol, apigenin and kaempferol triglucoside are substrates, as is genistein, which reacts more slowly, compounds with a hydroxy group in the 3' and 4' positions, such as quercetin and eriodictyol, do not act as substrates, similar to EC 2.1.1.75, apigenin 4'-O-methyltransferase and EC 2.1.1.83, 3,7-dimethylquercitin 4'-O-methyltransferase, ping-pong mechanism excluding the formation of a ternary complex | Dianthus caryophyllus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
callus | - |
Dianthus caryophyllus | - |
stem | - |
Dianthus caryophyllus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
70.5 | - |
pH 7, 25°C | Dianthus caryophyllus |
Storage Stability | Organism |
---|---|
-20°C, 2 weeks, 50% loss of activity | Dianthus caryophyllus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the role of F 4-OMT is mainly defensive against parasites, rather than structural | Dianthus caryophyllus | ? | - |
? | |
additional information | not: 4-hydroxybenzoic acid, gallic acid, p-coumaric acid, caffeic acid, caffeoyl-CoA, quercetin, rutin, luteolin, eriodictyol, 3,4,7-trihydroxyisoflavone, datiscetin | Dianthus caryophyllus | ? | - |
? | |
S-adenosyl-L-methionine + 4'-hydroxyflavanone | - |
Dianthus caryophyllus | S-adenosyl-L-homocysteine + 4'-methoxyflavanone | - |
? | |
S-adenosyl-L-methionine + a flavonoid | F 4-OMT specifically methylates the hydroxy substituent in 4-position of the flavones, flavanones and isoflavones in the presence of S-adenosyl-L-methionine, enzyme affinity for the substrate and catalytic efficiency decreases in the following order: 4-hydroxyflavones, 4-hydroxyflavanones, 4-hydroxyisoflavones, ping-pong mechanism, which excludes the formation of a ternary complex | Dianthus caryophyllus | S-adenosyl-L-homocysteine + a 4'-methoxyflavonoid | - |
? | |
S-adenosyl-L-methionine + apigenin | - |
Dianthus caryophyllus | S-adenosyl-L-homocysteine + acacetin | 4-methoxyapigenin | ? | |
S-adenosyl-L-methionine + genistein | - |
Dianthus caryophyllus | S-adenosyl-L-homocysteine + biochanin A | 4-methoxygenistein | ? | |
S-adenosyl-L-methionine + kaempferol | - |
Dianthus caryophyllus | S-adenosyl-L-homocysteine + kaempferide | - |
? | |
S-adenosyl-L-methionine + kaempferol 3-O-beta-D-glucopyranosyl-1,4-O-alpha-L-rhamnopyranosyl-1,2-beta-D-glucopyranoside | - |
Dianthus caryophyllus | S-adenosyl-L-homocysteine + 4'-O-methylkaempferol 3-O-beta-D-glucopyranosyl-1,4-O-alpha-L-rhamnopyranosyl-1,2-beta-D-glucopyranoside | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Dianthus caryophyllus |
Synonyms | Comment | Organism |
---|---|---|
F 4-OMT | - |
Dianthus caryophyllus |
flavonoid methyltransferase | - |
Dianthus caryophyllus |
flavonoid O-methyltransferase | - |
Dianthus caryophyllus |
methyltransferase, flavonoid | - |
Dianthus caryophyllus |
S-adenosyl-L-methionine:flavonoid 4-O-methyltransferase | - |
Dianthus caryophyllus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Dianthus caryophyllus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | 7 | - |
Dianthus caryophyllus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | 50% of maximum activity at pH 5.5 and 8.5, 4% of maximum activity at pH 5 | Dianthus caryophyllus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | 50% of maximum activity at pH 5.5 and 8.5, 4% of maximum activity at pH 5 | Dianthus caryophyllus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
S-adenosyl-L-homocysteine | pH 7, 25°C | Dianthus caryophyllus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Dianthus caryophyllus | isoelectric focusing, pH-gradient: 3-9 | - |
4.2 |